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| Catalog Number | Size | Price |
|---|---|---|
| LS-B7878-50 | 50 µg (0.5 mg/ml) | $460 |
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PathPlus™ Monoclonal Mouse anti‑Human PCNA Antibody (clone PC10 (a.k.a. 3F81), IHC, WB) LS‑B7878
PathPlus™ Monoclonal Mouse anti‑Human PCNA Antibody (clone PC10 (a.k.a. 3F81), IHC, WB) LS‑B7878
Note: This antibody replaces LS-C106804
Antibody:
PCNA Mouse anti-Human Monoclonal (PC10 (a.k.a. 3F81)) Antibody
Application:
IHC, IHC-P, WB, IP, Flo
Reactivity:
Human, Invertebrate, Vertebrate
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PCNA Mouse anti-Human Monoclonal (PC10 (a.k.a. 3F81)) Antibody
Application:
IHC, IHC-P, WB, IP, Flo
Reactivity:
Human, Invertebrate, Vertebrate
Format:
Unconjugated, Unmodified
Specifications
Description
PCNA (Proliferating cell nuclear antigen) is a replication factor that plays critical roles in DNA replication and repair. It is a cofactor of DNA polymerase delta, and acts as a homotrimer to increase the processivity of leading strand synthesis during DNA replication. It is ubiquitinated in response to DNA damage, and involved in the RAD6-dependent DNA repair pathway. Antibodies to PCNA are used as a marker of cell proliferation. It is expressed in the nucleus of proliferating cell populations.
References: Enzymes 2016 39:231; Nature Reviews Mol Cell Biol 2014 14:269
Host
Mouse
Reactivity
Human, Invertebrate, Vertebrate
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
PC10 (a.k.a. 3F81)
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Recombinant rat PCNA.
Applications
- IHC
- IHC - Paraffin (20 µg/ml)
- Western blot
- Immunoprecipitation
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The PC10 (a. k. a. 3F81) antibody has been tested by flow cytometric analysis. This can be used at less than or equal to 0.25 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Presentation
PBS, pH 7.2, 150 mM NaCl, 0.09% Sodium Azide
Storage
Store at 4°C, avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PCNA
Validation
Anti-PCNA / Cyclin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-PCNA / Cyclin antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-PCNA / Cyclin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
Anti-PCNA / Cyclin antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 20 ug/ml.
See More About...
LSBio Ratings
PathPlus™ PCNA Antibody (clone PC10 (a.k.a. 3F81)) for IHC, WB/Western, IP, Flow LS-B7878 has an LSBio Rating of
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
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Featured Products
Species:
Rat, Human, Mouse, Cat, Dog, Ferret, Hamster, Horse, Rabbit, Sheep, Chicken, Xenopus, Atlantic salmon, Monkey-Cynomolgus, Monkey-Rhesus
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Mouse, Rat, Pig
Applications:
IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Human, Monkey, Mouse, Rat, Bovine, Dog, Chicken, Xenopus, Fish
Applications:
IHC, IHC - Paraffin, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
