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| Catalog Number | Size | Price |
|---|---|---|
| LS-B90-0.2 | 0.2 ml (1 mg/ml) | $460 |
1 of 2
2 of 2
PathPlus™ Monoclonal Mouse anti‑Bovine RPE65 Antibody (clone 401.8B11.3D9, IHC, WB) LS‑B90
PathPlus™ Monoclonal Mouse anti‑Bovine RPE65 Antibody (clone 401.8B11.3D9, IHC, WB) LS‑B90
Note: This antibody replaces LS-C2878
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Target
Bovine RPE65
Synonyms
RPE65 | LCA2 | RBP-binding membrane protein | Retinol isomerase | SRPE65 | Rd12 | MRPE65 | Retinoid isomerohydrolase | RP20
Host
Mouse
Reactivity
Bovine, Human, Mouse, Rat, Dog, Pig, Chicken
(tested or 100% immunogen sequence identity)
Clonality
IgG1,k
Monoclonal
Clone
401.8B11.3D9
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Bovine RPE microsomal membrane proteins.
Specificity
RPE65.
Applications
- IHC - Paraffin (2.5 - 5 µg/ml)
- ICC
- Western blot (1:5000 - 1:10000)
- Flow Cytometry
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B90 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B90 was determined to be 2.5 ug/ml.
Presentation
PBS, 0.02% Sodium Azide
Storage
Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About RPE65
Validation
Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Retinal Epithelium: Formalin-Fixed, Paraffin-Embedded (FFPE)
Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-RPE65 antibody IHC of human brain cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
See More About...
LSBio Ratings
PathPlus™ RPE65 Antibody (clone 401.8B11.3D9) for IHC, ICC, WB/Western, Flow LS-B90 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (5)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Endogenous regeneration of damaged retinal pigment epithelium following low dose sodium iodate administration: an insight into the role of glial cells in retinal repair. Machali[Character 144]ska A, Kawa MP, Pius-Sadowska E, Rogi[Character 144]ska D, K[Character 142]os P, Baumert B, Wiszniewska B, Machali[Character 144]ski B. Experimental eye research. 2013 Jul;112:68-78.
Customer Reviews (0)
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Species:
Human, Rat
Applications:
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Species:
Human, Monkey, Rat, Bovine, Dog, Horse, Pig, Rabbit
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Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Species:
Bovine, Human, Mouse, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Bovine, Human, Mouse, Pig
Applications:
IHC, IHC - Paraffin, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Bovine, Human, Mouse, Rat, Pig
Applications:
IHC, IHC - Frozen, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human, Rat
Applications:
IHC - Paraffin, Immunofluorescence, Western blot, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
Date Requested: 11/3/2024
