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Catalog Number Size Price
LS-B5909-50 50 µg (0.5 mg/ml) $485 
VPS35 Antibody - Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
VPS35 Antibody - VPS35 / MEM3 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
VPS35 Antibody - VPS35 / MEM3 antibody staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
VPS35 Antibody - VPS35 / MEM3 antibody staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
VPS35 Antibody - VPS35 / MEM3 antibody (1µg/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
VPS35 Antibody - Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
VPS35 Antibody - VPS35 / MEM3 antibody immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
VPS35 Antibody - VPS35 / MEM3 antibody staining of PFA-fixed and saponin permeabilized SHSY5Y and detected with FITC in confocal microscopy. Data obtained from Dr. M. Schallburg Nielsen, Aarhus University Denmark.
VPS35 Antibody - VPS35 / MEM3 antibody staining of PFA-fixed and saponin permeabilized HEK293 and detected with FITC in confocal microscopy. Data obtained from Aarhus University Denmark.
VPS35 Antibody - VPS35 / MEM3 antibody (1µg/ml) staining of Human Brain (Cerebellum) (A) and Rat Brain (B) lysates (35µg protein in RIPA buffer). Detected by chemiluminescence.
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PathPlus™ Polyclonal Goat anti‑Human VPS35 Antibody (aa783‑796, IHC, IF, WB) LS‑B5909

PathPlus™ Polyclonal Goat anti‑Human VPS35 Antibody (aa783‑796, IHC, IF, WB) LS‑B5909

Note: This antibody replaces LS-C55677
Antibody:
VPS35 Goat anti-Human Polyclonal (aa783-796) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B5909-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
VPS35 Goat anti-Human Polyclonal (aa783-796) Antibody
Application:
IHC, IHC-P, IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
VPS35 is a Vacuolar Protein Sorting protein that is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. Inherited mutations in VPS35 are linked with neurodegeneration and Parkinson's disease, likely through mitochondrial dysfunction resulting from increased interaction of mutant VPS35 with DLP1. In immunohistochemistry, VPS35 has moderate to high cytoplasmic positivity in all tissues throughout the body. References: Nat Med. 2016 Jan;22(1):54-63, PMID: 26618722;
Target
Human VPS35
Synonyms
VPS35 | MEM3 | Maternal-embryonic 3 | Vesicle protein sorting 35 | PARK17 | HVPS35
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-SPESEGPIYEGLIL, from the C-Terminus of protein sequence according to NP_060676.2.
Epitope
aa783-796
Specificity
Human VPS35. Note there is a hypothetical protein called similar to vacuolar protein sorting 35 (XP_040192.1), which is virtually identical.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About VPS35
Q96QK1 NM_018206 NP_060676.2

Validation

VPS35 Antibody - Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-VPS35 antibody IHC of human breast, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

PathPlus™ VPS35 Antibody (aa783-796) for IHC, IF/Immunofluorescence, WB/Western LS-B5909 has an LSBio Rating of
Publications (4.1)
Laboratory Validation Score (5)

Publications (2)

Rab GTPase-activating proteins in autophagy: regulation of endocytic and autophagy pathways by direct binding to human ATG8 modifiers. Popovic D, Akutsu M, Novak I, Harper JW, Behrends C, Dikic I. Molecular and cellular biology. 2012 32:1733-44. (WB; Human)

Novel Insights into the Cellular Localization and Regulation of the Autophagosomal Proteins LC3A, LC3B and LC3C. Marius W Baeken, Katja Weckmann, Philip Diefenthäler, Jan Schulte, Kamran Yusifli, Bernd Moosmann, Christian Behl, Parvana Hajieva. Cells. 2020 October;9:

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/24/2024