ELISA Kit Reagents and Accessories

LSBio offers all the reagents and accessories you’ll need in order to build a better ELISA assay. Our catalog of coating buffers, blocking buffers, wash buffers, substrates, diluents, and much more allow the most flexibility for your research needs. Our reagents are specifically formulated to help you achieve the best signal while decreasing background noise. Explore the reagents and accessories we have that will help build your ELISA assay every step of the way.

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Other (31)

Assay Kits (2)

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ELISA Kit Component (2)

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Blocking Buffer (6)

Coating Buffer (2)

Conjugates and Stabilizers (5)

ELISA Kit Reagents and Accessories (33)

Sample and Assay Diluent (8)

Stop Solution (2)

Substrates (6)

Wash Buffer (2)

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Antibody Pair Support Pack 1 - LS-K1087

Target:

ELISA Kit Component

Price:

5 Plates/$93; 10 Plates/$155

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Antibody Pair Support Pack 2 - LS-K1088

Target:

ELISA Kit Component

Price:

5 Plates/$90; 10 Plates/$145

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ELISA Blocking Buffer (Non-Mammalian) - LS-M17

Description:

ELISA Blocking Buffer (Non-Mammalian) is designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. This buffer is particularly useful when working with human and other mammalian serum samples as it works to reduce interactions between sample and blocking molecules.

Price:

100 ml/$66; 500 ml/$185; 1 l/$329

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General ELISA Assay Diluent - LS-M19

Description:

General Assay Diluent is a mammalian protein-based ELISA additive used to equalize the sample and standard matrices for a more accurate result. Large differences in matrix complexity environment between standard curve and sample wells are the main cause for target analyte under-recovery problems in sandwich ELISA analysis. By helping to equalize matrix complexity, General Assay Diluent reduces non-specific interactions and interference.

Price:

500 ml/$228; 1 l/$295; 100 ml/$68

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ELISA IgM-Reducing Assay Diluent - LS-M20

Description:

IgM-Reducing Assay Diluent is a specially formulated additive to address IgM-mediated conjugate bridging interference in problematic serum and plasma samples tested in antibody sandwich ELISA formats. IgM-Reducing Assay Diluent can be added to standard curve/calibrator and serum/plasma sample wells to help equalize matrix complexity disparity issues.

Price:

500 ml/$240; 1 l/$306; 100 ml/$68

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ELISA Assay Diluent (Non-Mammalian) - LS-M21

Description:

Assay Diluent (Non-Mammalian) is formulated to address nonspecific binding issues with human, porcine, or bovine serum or plasma samples tested in antigen-down ELISA formats. This non-mammalian assay additive works to enhance the specific anti-analyte/antigen antibody signal without denaturing the plate-adsorbed antigen molecules.

Price:

100 ml/$68; 500 ml/$240; 1 l/$306

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ELISA Assay Diluent (Antigen-Down) - LS-M22

Description:

ELISA Assay Diluent (Antigen-Down) is formulated for use with serum and plasma samples tested in antigen-down ELISA formats. Proprietary additives have been included to reduce the level of nonspecific IgG adsorption to antigen-coated and blocked ELISA well surfaces, as well as enhancing the specific anti-analyte/antigen-antibody signal without denaturing the plate adsorbed antigen molecules.

Price:

500 ml/$245; 1 l/$372; 100 ml/$68

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ELISA Blocking Buffer (General) - LS-M23

Description:

ELISA Blocking Buffer (General) contains a mammalian protein blocking agent suitable for most antibody capture ELISA formats and peptide or protein antigen-down ELISA formats. This blocking buffer provides a long-term stabilizing environment for dried antigen or antibody coat proteins and minimizes nonspecific binding interactions during the assay process.

Price:

100 ml/$60; 500 ml/$145

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ELISA Blocking Buffer (Background Blocking) - LS-M24

Description:

Eliminate interference and nonspecific background noise associated with antibody-coated ELISA formats and sandwich ELISAs. ELISA Blocking Buffer (Background Blocking) provides superior background-blocking performance without the use of conventional cross-reactive protein additives.

Price:

100 ml/$64; 500 ml/$160; 1 l/$291

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ELISA Antibody Coating Buffer, 5X - LS-M25

Description:

Use Antibody Coating Buffer to save reagents, enhance specific signal, and extend the shelf life of coated plates. Antibody Coating Buffer maximizes the adsorption of monoclonal and polyclonal antibodies onto polystyrene plates for use in capture (sandwich) immunoassays by stabilizing the three-dimensional structure of the antibody for optimal immunoassay performance.

Price:

500 ml/$166; 1 l/$318; 100 ml/$66

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General ELISA Serum Diluent - LS-M26

Description:

General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. General Serum Diluent can also be used for dilution of many types of serum samples, including mammalian and chicken, tested in antigen-down ELISA formats.

Price:

500 ml/$228; 1 l/$295; 100 ml/$68

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ELISA Wash Buffer, 10X - LS-M27

Description:

ELISA Wash Buffer, 10X effectively rinses microtiter plates between reagent addition steps of an ELISA to remove signal-altering debris yet preserve assay components.

Price:

500 ml/$135; 1 l/$257; 100 ml/$60

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HRP Conjugate Stock Stabilizer, 5X - LS-M28

Description:

HRP Conjugate Stock Stabilizer is best for users who desire an all-purpose HRP conjugate diluent and HRP stabilizer. This formulation may serve as a base to add specialized components to address non-specific binding and interference. It also serves as an excellent concentrated buffer to store HRP conjugates.

Price:

500 ml/$586; 1 l/$1,102; 100 ml/$122

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ELISA Blocking Buffer (High Strength) - LS-M48

Description:

ELISA Blocking Buffer (High Strength) is a high strength blocking buffer designed to address high background problems in antigen-down and sandwich immunoassays. Using a heterogenous mixture of non-mammalian blocking agents, ELISA Blocking Buffer (High Strength) reduces non-specific binding and stabilizes proteins.

Price:

100 ml/$64; 500 ml/$146; 1000 ml/$291

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ELISA Blocking Buffer (Synthetic) - LS-M49

Description:

ELISA Blocking Buffer (Synthetic) provides superior background-blocking performance to eliminate interference and minimize background noise on ELISA formats without the use of conventional cross-reactive protein additives.

Price:

500 ml/$146; 1 l/$291; 100 ml/$64

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HRP Conjugate Stabilizer, Mammalian, 1X - LS-M50

Description:

Preserve activity of HRP conjugates and reduce background signal in immunoassays using HRP detection systems. HRP Conjugate Stabilizer is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques.

Price:

1000 ml/$234; 100 ml/$75

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ELISA Protein-Free Sample Diluent - LS-M51

Description:

Protein-Free Sample Diluent is a protein-free matrix used for the dilution of biological samples (e.g. serum, cell culture media) into the useful range of antibody-sandwich or antigen-down ELISA format assays. This unique buffer contains a heterogeneous mixture of proprietary molecules that help reduce background noise associated with non-specific bridging of signal-generating conjugates to the plate well surface.

Price:

100 ml/$61; 500 ml/$143; 1000 ml/$258

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HRP Conjugate Stabilizer, Non-Mammalian, 1X - LS-M52

Description:

Preserve activity of HRP conjugates and reduce background signal. HRP Conjugate Stabilizer, Non-Mammalian is used to preserve concentrated stock conjugates, reconstitute lyophilized conjugates, and dilute antibody-HRP conjugates to their useful working titer in ELISAs and immunology-based techniques. This product is best for immunoassays using anti-IgG HRP conjugates within traditional antigen-down or antibody sandwich ELISA formats.

Price:

1000 ml/$234; 500 ml/$117; 100 ml/$75

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TMB Substrate (Low Sensitivity) - LS-M38

Description:

TMB Substrate (Low Sensitivity) is a ready-to-use solution containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer.

Price:

100 ml/$76

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pNPP Substrate - LS-M39

Description:

pNPP Substrate with Stabilizing Pellets is a ready-to-use alkaline phosphatase substrate formulation to detect alkaline phosphatase-conjugated molecules in ELISA and microwell assays.

Price:

100 ml/$69

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TMB Substrate (Immunoblotting) - LS-M40

Description:

TMB Substrate (Immunoblotting) is a ready-to-use formulation containing 3,3',5,5'-tetramethylbenzidine in a mildly acidic buffer.

Price:

100 ml/$82

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BCIP/NBT Substrate - LS-M41

Description:

BCIP/NBT Substrate is useful for immunoblotting applications where alkaline phosphatase-conjugated molecules are used for detection. NBT serves as the oxidant and BCIP is the AP substrate. BCIP/NBT Substrate reacts with AP yielding a dark blue reaction product.

Price:

100 ml/$82

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ELISA Stop Solution - LS-M43

Description:

Stop Solution is a ready-to-use liquid acidic stop solution suitable for use with our TMB substrates. This product is suitable for all endpoint ELISAs using a TMB substrate for color development.

Price:

100 ml/$46

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ELISA Stop Solution (AP) - LS-M44

Description:

Stop Solution (AP)is a dry powder for use with pNPP-based colorimetric substrates. This product is suitable for all endpoint ELISAs using an AP substrate for color development of an AP-conjugate.

Price:

100 ml/$51

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ELISA Plasma Sample Diluent (Antigen-Down) - LS-M29

Description:

ELISA Plasma Sample Diluent (Antigen-Down) is formulated specifically for use with plasma samples being tested in an antigen-down ELISA format. This novel mammalian-protein-buffered formulation provides an assay environment that minimizes non-specific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction.

Price:

100 ml/$68; 500 ml/$228; 1 l/$295

Viewing 1-25 of 33 product results

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