Target
CCL2 / MCP1
Synonyms
CCL2 | C-C motif chemokine 2 | Chemokine (C-C motif) ligand 2 | GDCF-2 | HC11 | HSMCR30 | MCP1 | SCYA2 | Small-inducible cytokine A2 | Monocyte secretory protein JE | MCAF | MCP-1 | Monocyte chemotactic protein 1 | SMC-CF
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 76 amino acid recombinant protein has a predicted molecular mass of approximately 8,685 Da. The DTT-reduced protein migrates at approximately 8kDa and non-reduced protein migrates at approximately 13kDa by SDS-PAGE. The N-terminal amino acid is Glycin
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Recombinant human CCL2 (MCP-1) chemoattracts Baf3-mCCR2 transfectants in a dose-dependent manner. The ED50 for this effect is 0.3 – 1.8 ng/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution, pH 7.2, 0.15 M NaCl, 10 mM NaHPO4
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee