Target
CD47
Synonyms
CD47 | CD47 antigen | CD47 molecule | Integrin associated protein | Integrin-associated protein | Leukocyte surface antigen CD47 | MER6 | Protein MER6 | Rh-related antigen | OA3 | CD47 glycoprotein | IAP
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant human CD47 consists of 132 amino acids and predicts a molecular mass of 15.2 kDa.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human CD47-His at 10µg/mL (100µL/well) can bind biotinylated human SIRPA-His, the EC50 of biotinylated human SIRPA-His is 0.2-0.5µg/mL.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS,pH7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee