Target
CD47
Synonyms
CD47 | CD47 antigen | CD47 molecule | Integrin associated protein | Integrin-associated protein | Leukocyte surface antigen CD47 | MER6 | Protein MER6 | Rh-related antigen | OA3 | CD47 glycoprotein | IAP
Conjugations
Unconjugated
Tag
Human IgG1 Fc, C-terminus
Predicted Molecular Weight
60-65 kDa
AA Sequence
QLLFNKTKSVEFTFCNDTVVIPCFVTNMEAQNTTEVYVKWKFKGRDIYTFDGALNKSTVPTDFSSAKIEVSQLLKGDASLKMDKSDAVSHTGNYTCEVTELTREGETIIELKYRVVSWFSPexpressedwithaC-terminalhumanIgG1Fcfragment
Expression System
HEK 293 Cells
Purification
Greater than 97% by SDS-PAGE
Bio-Activity
Immobilized SIRPa-His, Human (Cat.No.Z03421) at 2µg/mL (100 µl/well), can bind CD47 Fc Chimera, Human (Z03418) with a linear range of 0.25-185 ng/mL.
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered solution in PBS
Reconstitution
Reconstitute in ddH2O or PBS at 100 ug/ml.
Storage
Store lyophilized at -70°C or below for up to 6 months. Store reconstituted at 4°C for up to 1 week or at -20°C for up to 3 months. Add a carrier protein (0.1% BSA) for long term storage. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee