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| Catalog Number | Size | Price |
|---|---|---|
| LS-G137845-10 | 10 µg (0.2 mg/ml) | $355 |
| LS-G137845-25 | 25 µg (0.2 mg/ml) | $497 |
Human GZMB / Granzyme B Protein (Recombinant) - LS-G137845
Human GZMB / Granzyme B Protein (Recombinant) - LS-G137845
Description:
GZMB / Granzyme B Protein LS-G137845 is a Recombinant Human GZMB / Granzyme B produced in HEK 293 Cells. It is low in endotoxin; Less than 1 EU/µg (determined by LAL method). For Research Use Only
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(800) 227-6666
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Overview
Description:
GZMB / Granzyme B Protein LS-G137845 is a Recombinant Human GZMB / Granzyme B produced in HEK 293 Cells. It is low in endotoxin; Less than 1 EU/µg (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
GZMB / Granzyme B
Synonyms
GZMB | CCPI | CGL-1 | Cathepsin G-like 1 | CSP-B | CTLA-1 | CTLA1 | CGL1 | CSPB | Fragmentin 2 | GRB | HLP | Human lymphocyte protein | Fragmentin-2 | SECT | Lymphocyte protease | T-cell serine protease 1-3E | C11 | CTSGL1 | Cytotoxic serine protease B | Granzyme B | Granzyme-2
Species
Human
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
This 244 amino acid recombinant protein has a predicted molecular mass of approximately 27.5 kD. The protein migrates at about 37 kD in DTT-reducing conditions and about 37 kD in non-reducing conditions by SDS-PAGE.The predicted N-terminal amino acid is G
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
Human Granzyme B activated by mouse Cathepsin C is able to cleave the peptide substrate t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >1500 pmol/min/µg. This recombinant protein is in its inactive form and needs to be activated prior to use in bioassays. Please contact technical support for a detailed activation protocol.
Endotoxin
Less than 1 EU/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 20 mM Tris, pH 7.5, 150 mM NaCl
Storage
Store unopened at -70°C for up to 6 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GZMB / Granzyme B
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Functional Assay
The activity of recombinant human Granzyme B is determined by its ability to cleave a colorimetric peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >1500 pmol/min/µg.
Functional Assay
The activity of recombinant human Granzyme B is determined by its ability to cleave a colorimetric peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >1500 pmol/min/µg.
Functional Assay
The activity of recombinant human Granzyme B is determined by its ability to cleave a colorimetric peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >1500 pmol/min/µg.
Functional Assay
The activity of recombinant human Granzyme B is determined by its ability to cleave a colorimetric peptide substrate, t-Butyloxycaronyl-Ala-Ala-Asp-ThioBenzyl ester (Boc-AAD-SBzl), in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity >1500 pmol/min/µg.
Popular GZMB / Granzyme B Proteins
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/22/2024
Date Requested: 12/22/2024
