Target
IL21
Synonyms
IL21 | Interleukin 21 | Interleukin-21 | Interleukin-21 isoform | Za11 | IL-21
Modifications
Carrier-free
Conjugations
Unconjugated
Predicted Molecular Weight
The 133 amino acid recombinant protein has a predicted molecular mass of approximately 15.5 kD. The DTT-reduced and non-reduced protein migrate at approximately 16 kD by SDS-PAGE. The N-terminal amino acid is Glutamine.
Purification
Greater than 98% by SDS-PAGE
Usage Summary
Stock solutions can also be prepared at 50-100 µg/mL in sterile buffer (PBS, HPBS, DPBS, or EBSS) containing carrier protein such as 0.2-1% BSA or HSA.
Bio-Activity
Recombinant human IL-21 induces the proliferation of mouse HT-2 cells in a dose-dependent manner. The ED50 for this effect is 0.05 - 0.5 µg/mL.
Endotoxin
Less than 0.01 ng/µg (determined by LAL method).
Presentation
0.22 µm filtered protein solution in 1x PBS, 1 mM EDTA
Storage
Store unopened at 2°C to 8°C for up to 1 month, at -20°C for up to 6 months, or at -70°C for up to 1 year. Store diluted at -20°C to -70°C. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee