Target
IL5
Synonyms
IL5 | EDF | Interleukin-5 | IL-5 | T-cell replacing factor | TRF
Modifications
Animal-free
Conjugations
Unconjugated
Predicted Molecular Weight
26.5 kDa
AA Sequence
MIPTEIPTSALVKETLALLSTHRTLLIANETLRIPVPVHKNHQLCTEEIFQGIGTLESQTVQGGTVERLFKNLSLIKKYIDGQKKKCGEERRRVNQFLDYLQEFLGVMNTEWIIES
Expression System
E. coli
Purification
Greater than 98% by SDS-PAGE gel and HPLC analyses.
Bio-Activity
The ED50 as determined by the dose-dependent proliferation of TF-1 cells was = 0.15 ng/ml, corresponding to a specific activity of = 6.6 x 10^6 units/mg.
Endotoxin
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
Presentation
Lyophilized from sterile filtered 10mM Tris, pH 8.5, 5mM NaCl
Reconstitution
Centrifuge vial prior to opening. Do not vortex. Reconstitute with H2O to 0.1-1.0 mg/ml.
Storage
Before reconstitution: Short term: Room temperature; Long term: -20°C to -80°C. After reconstitution: Short term: 4°C; Long term: Aliquot and store with a carrier protein at -20°C to -80°C. Avoid freeze thaw cycles. Microcentrifuge before opening.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee