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Proteins > LAG3 > LS-G3890
 
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Catalog Number Size Price
LS-G3890-50 50 µg $442 
LAG3 Protein - Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.
LAG3 Protein - LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
LAG3 Protein - Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.
LAG3 Protein - LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
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Human LAG3 Protein (Recombinant Human IgG1 Fc) - LS-G3890

Human LAG3 Protein (Recombinant Human IgG1 Fc) - LS-G3890

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Description:
LAG3 Protein LS-G3890 is a Recombinant Human LAG3 produced in CHO Cells with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$442
LS-G3890-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Publications Reviews Images Popular LAG3 Proteins Request SDS/MSDS

Overview

Description:
LAG3 Protein LS-G3890 is a Recombinant Human LAG3 produced in CHO Cells with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
LAG3
Synonyms
LAG3 | CD223 antigen | LAG-3 | Protein FDC | CD223 | FDC | Lymphocyte-activation gene 3
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Predicted Molecular Weight
~80kDa (SDS-PAGE)
Expression System
CHO Cells
Source Species
Hamster-Chinese
Purification
Greater than 99% by SDS-PAGE
Bio-Activity
Inhibits binding of MAb to LAG-3 (human) to LAG-3. Induces maturation of human dendritic cells.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
PBS
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 6 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About LAG3
P18627 NM_002286 P18627

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Images

Functional Assay

LAG3 Protein - Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.
Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.

Functional Assay

LAG3 Protein - LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.

Functional Assay

LAG3 Protein - Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.
Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.

Functional Assay

LAG3 Protein - LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.

Functional Assay

LAG3 Protein - Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.
Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.

Functional Assay

LAG3 Protein - LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.

Functional Assay

LAG3 Protein - Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.
Binding of LAG-3 (human):Fc (human) (rec.) and EBV-transformed B-cells are incubated with either an anti-MHC class II MAb (10 ug/ml) or LAG-3 (human):Fc (human) (rec.) (10 ug/ml) for 30 minutes. at 4°C in PBS/1% BSA. The cells are then stained for 30 minutes. at 4°C with FITC-labeled goat anti-human Fc antibodies.

Functional Assay

LAG3 Protein - LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.
LAG 3 (human):Fc (human) (rec.)-induced dendritic cells (DC) maturation. Method: Immature DCs derived from human monocytes, resuspended at 1x10^6 cells/ml in complete culture medium, are incubated with LAG-3 (human):Fc (human) (rec.) (10ug/ml), LPS (positive control, 5ug/ml) or human IgG1 (isotype-matched negative control, 10ug/ml) for 48 hours. DC maturation induced by LAG-3 (human):Fc (human) (rec.) binding to MHC class II or LPS is assessed by the increased expression of MHC Class II, CD80, CD83, CD86 and CD40.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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United States

Tel: (800) 227-6666 / (650) 697-3600

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