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| Catalog Number | Size | Price |
|---|---|---|
| LS-G48836-100 | 100 µg | $480 |
| LS-G48836-1 | 1 mg | $2,333 |
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Human OSM / Oncostatin M Protein (Recombinant His,C-terminus) (Met1-Arg221) - LS-G48836
Human OSM / Oncostatin M Protein (Recombinant His,C-terminus) (Met1-Arg221) - LS-G48836
Description:
OSM / Oncostatin M Protein LS-G48836 is a Recombinant Human OSM / Oncostatin M produced in HEK 293 Cells Met1-Arg221 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
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(800) 227-6666For Research Use Only
Overview
Description:
OSM / Oncostatin M Protein LS-G48836 is a Recombinant Human OSM / Oncostatin M produced in HEK 293 Cells Met1-Arg221 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
OSM / Oncostatin M
Synonyms
OSM | Oncostatin-M | Oncostatin M
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Arg221
Predicted Molecular Weight
The recombinant human OSM consists of 207 amino acids and has a predicted molecular mass of 23.6 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhOSM is approximately 32-35 kDa due to glycosylation.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA. Immobilized human OSM-His at 10 µg/ml (100 µl/well) can bind biotinylated human LIFR-His with a linear range of 0.031-0.5 µg/ml. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2.5 ng/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About OSM / Oncostatin M
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Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2.5 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2.5 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2.5 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-2.5 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular OSM / Oncostatin M Proteins
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
Date Requested: 12/21/2024
