Recombinant Protein

RBP4

Human

Unmodified

22 kDa

HEK 293 Cells

Human

Greater than 97% by SDS-PAGE and RP-HPLC

Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. >1.0 µM all-trans retinoic acid is bound under the described conditions. Assay Protocol: 1. Dilute rhRBP4 to 49 µg/mL in Assay Buffer. 2. Make serial dilutions of retinoic acid in 95% ethanol at 100, 30, 10, 3 and 1 µM. 3. The formation of Protein/Retinol binding in microtubes: 3.1. Mix 112.5 µL of 49 µg/mL rhRBP4 and 12.5 µL of retinoic acid serial dilutions in microtubes. 3.2. For a blank, mix 112.5 µL of 49 µg/mL rhRBP4 and 12.5 µL of 95% ethanol in a microtube. 4. Incubate reaction tubes at room temperature for 30 minutes. 5. In a plate load 100 µL of the reaction mixtures and blank. 6. Read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. 7. Calculate concentration at which 50% quenching of rhRBP4 is achieved by plotting raw RFUs vs. concentration of retinoic acid with 4-PL fitting. Use th

Less than 0.2 EU/µg protein (determined by LAL method).

Lyophilized from a 0.2 um filtered solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl

Reconstitute in ddH2O or PBS at 100 ug/ml.

Store lyophilized at -70°C or below for up to 6 months. Store reconstituted at 4°C for up to 1 week or at -20°C for up to 3 months. Add a carrier protein (0.1% BSA) for long term storage. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P02753 NCBI: NM_006744 NP_006735.2