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| Catalog Number | Size | Price |
|---|---|---|
| LS-G27306-10 | 10 µg | $262 |
| LS-G27306-50 | 50 µg | $304 |
| LS-G27306-1 | 1 mg | $990 |
Human TNF Alpha Protein (Recombinant) - LS-G27306
Human TNF Alpha Protein (Recombinant) - LS-G27306
Description:
TNF Alpha Protein LS-G27306 is a Recombinant Human TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.2 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G27306 is a Recombinant Human TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.2 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Predicted Molecular Weight
17.3 kDa (SDS-PAGE)
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
ED50 < 30 pg/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 3.3 x 10^7 units/mg.
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Storage
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 1 week or at -20°c for up to 3 months.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
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Enzyme-Linked Immunosorbent Assay
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Enzyme-Linked Immunosorbent Assay
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Enzyme-Linked Immunosorbent Assay
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Enzyme-Linked Immunosorbent Assay
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/20/2024
Date Requested: 11/20/2024
