Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
Products
Antibodies
ELISA and Assay Kits
Research Areas
Infectious Disease
Resources
Purchasing
Reference Material
Contact Us
Location
Corporate Headquarters
Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
United States
Telephone Numbers
Customer Service: (800) 227-6666 / (650) 697-3600
Contact Us
Additional Contact Details
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Forgot password?
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.
order histories, retained contact details for faster checkout, review submissions, and special promotions.
Quick Order
| Catalog Number | Size | Price |
|---|---|---|
| LS-G47212-10 | 10 µg | $255 |
| LS-G47212-100 | 100 µg | $364 |
| LS-G47212-1 | 1 mg | $1,452 |
1 of 3
2 of 3
3 of 3
Human TNF Alpha Protein (Recombinant) (Val77-Leu233) - LS-G47212
Human TNF Alpha Protein (Recombinant) (Val77-Leu233) - LS-G47212
Description:
TNF Alpha Protein LS-G47212 is a Recombinant Human TNF Alpha produced in E. coli Val77-Leu233. It is biologically active. For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G47212 is a Recombinant Human TNF Alpha produced in E. coli Val77-Leu233. It is biologically active. For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Region
Val77-Leu233
Predicted Molecular Weight
The recombinant human TNF-a consists of 158 amino acids and has a predicted molecular mass of 17.4 kDa which is also estimated by SDS-PAGE.
Expression System
E. coli
Source Species
E. coli
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 5-50 pg/mL.
Endotoxin
Not Tested
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
Publications (0)
Customer Reviews (0)
Images
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Labeled biotin to TNF-alpha Protein, Human, Recombinant by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of TNF-alpha Protein, Human, Recombinant, Biotinylated(Cat. 10602-HNAE-B) bound to Humira was 0.1 nM.
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 5-50 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/20/2024
Date Requested: 11/20/2024
