Recombinant Protein

EPO / Erythropoietin

Mouse

Unmodified

The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.

HEK 293 Cells

Human

> 96 % as determined by SDS-PAGE

Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.

Less than 1.0 EU/µg protein (determined by LAL method).

Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.

Please refer to included instructions for detailed information.

Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P01588 NCBI: NM_000799 NP_000790.2