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| Catalog Number | Size | Price |
|---|---|---|
| LS-G48956-50 | 50 µg | $403 |
1 of 2
2 of 2
Mouse EPO / Erythropoietin Protein (Recombinant His,C-terminus) (Met1-Arg192) - LS-G48956
Mouse EPO / Erythropoietin Protein (Recombinant His,C-terminus) (Met1-Arg192) - LS-G48956
Description:
EPO / Erythropoietin Protein LS-G48956 is a Recombinant Mouse EPO / Erythropoietin produced in HEK 293 Cells Met1-Arg192 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
EPO / Erythropoietin Protein LS-G48956 is a Recombinant Mouse EPO / Erythropoietin produced in HEK 293 Cells Met1-Arg192 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
EPO / Erythropoietin
Synonyms
EPO | EP | MVCD2 | Erythropoietin
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Arg192
Predicted Molecular Weight
The recombinant mouse EPO comprises 177 amino acids and has a predicted molecular mass of 20 kDa. The apparent molecular mass of the protein is approximately 37 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 96 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to bind biotinylated human EPOR-His in a functional ELISA. Measured by its ability to bind mouse EPOR-Fch in a functional ELISA. Measured by its ability to bind human EPOR & CD131-Fc in a functional ELISA. Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About EPO / Erythropoietin
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Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Proliferation
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is typically 0.5-3 ng/ml.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular EPO / Erythropoietin Proteins
Source:
E. coli
Tag:
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Source:
Wheat
Tag:
GST
Source:
Hamster-Chinese
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Human Mutant IgG1 Fc
Source:
E. coli
Tag:
His-SUMO
Cynomolgus monkey EPO / Erythropoietin Protein (Recombinant 6His, N-terminus) (aa28-192) - LS-G22910
Source:
Yeast
Tag:
6His, N-terminus
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/4/2024
Date Requested: 11/4/2024
