Recombinant Protein

GZMB / Granzyme B

Mouse

Carrier-free

This 246 amino acid recombinant protein has a predicted molecular mass of approximately 27.5 kD. The protein migrates at approximately 37 kD in DTT-reducing conditions and approximately 37 kD in non-reducing conditions by SDS-PAGE. The predicted N-termina

HEK 293 Cells

Human

Greater than 95% by SDS-PAGE

The recombinant protein could be aliquoted and stored at -20°C in a sterile buffer (20 mM HEPES, 150 mM NaCl, and pH 7.4).

Recombinant mouse Granzyme B supplied in its inactive form. Once the mouse Granzyme B is activated by active mouse Capthepsin C/DPPI, it is able to cleave the peptide substrate t-Butyloxycaronyl-Ala-Ala-ThioBenzyl ester (Boc-AAD-SBzl) in the presence of 5,5'Dithio-bis (2-nitrobenzoic acid) (DTNB), with an activity > 3000 pmol/min/µg.

Less than 1 EU/µg (determined by LAL method).

0.22 µm filtered protein solution in 20 mM HEPES, pH 7.4, 150 mM NaCl

Store unopened at -70°C for up to 6 months. Avoid freeze/thaw cycles. For maximum results, quick spin vial prior to opening.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P10144 NCBI: NM_004131 NP_004122.2