Recombinant Protein

IL-10

Mouse

Unmodified

18.7 kDa

E. coli

E. coli

Greater than 98% by SDS-PAGE and HPLC

This Murine IL-10 is fully biologically active as measured by the dose-dependent co-stimulation (with IL-4) of the proliferation of murine MC/9 cells. The ED50 was found to be <2 ng/ml, corresponding to a specific activity of >5 x 10^5 units/mg.

Less than 0.1 ng/µg of protein (less than 1EU/µg).

Sterile filtered, lyophilized from 5 mM sodium phosphate, pH 8.0

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Note: Allow the reconstituted vial to sit at room temperature for 1 hour before use. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This protein carries the LSBio 100% Guarantee.

Uniprot: P22301 NCBI: NM_000572 NP_000563.1