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| Catalog Number | Size | Price |
|---|---|---|
| LS-G49419-20 | 20 µg | $317 |
| LS-G49419-100 | 100 µg | $480 |
| LS-G49419-1 | 1 mg | $2,344 |
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2 of 2
Mouse TNF Alpha Protein (Recombinant) (Leu80-Leu235) - LS-G49419
Mouse TNF Alpha Protein (Recombinant) (Leu80-Leu235) - LS-G49419
Description:
TNF Alpha Protein LS-G49419 is a Recombinant Mouse TNF Alpha produced in E. coli Leu80-Leu235. It is biologically active. For Research Use Only
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G49419 is a Recombinant Mouse TNF Alpha produced in E. coli Leu80-Leu235. It is biologically active. For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Region
Leu80-Leu235
Predicted Molecular Weight
The recombinant mouse TNF-a consists of 157 amino acids and migrates with an apparent molecular mass of 17 kDa as estimated in SDS-PAGE under reducing conditions.
Expression System
E. coli
Source Species
E. coli
Purification
> 98 % as determined by SDS-PAGE
Bio-Activity
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Endotoxin
Not Tested
Presentation
Lyophilized from sterile 50mM Tris 0.2M NaCl, pH 7.2, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
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Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 3-30 pg/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
Date Requested: 12/26/2024
