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ACLY / ATP Citrate Lyase

ATP citrate lyase

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

Gene Name: ATP citrate lyase
Synonyms: ACLY, ACL, ATP citrate synthase, ATPCL, ATP citrate lyase, ATP-citrate (pro-S-)-lyase, CLATP, ATP-citrate synthase, Citrate cleavage enzyme, Citrate Lyase
Target Sequences: NM_001096 NP_001087.2 P53396

Publications (2)

1
Timosaponin AIII is preferentially cytotoxic to tumor cells through inhibition of mTOR and induction of ER stress. King FW, Fong S, Griffin C, Shoemaker M, Staub R, Zhang YL, Cohen I, Shtivelman E. PloS one. 2009 4:e7283. (WB) [PubMed:19789631] [PMC:PMC2747272]
2
Hypoxia further exacerbates woody breast myopathy in broilers via alteration of satellite cell fate. Nima K Emami, Reagan N Cauble, Ahmed E Dhamad, Elizabeth S Greene, Cynthia S Coy, Sandra G Velleman, Sara Orlowski, Nicholas Anthony, Mike Bedford, Sami Dridi. Poultry science. 2021 Jul;;100:101167. [Full Text Article] [PubMed:34091348] [PMC:PMC8182261]

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Proteins (5)
Over-Expression Lysate (2)
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ACLY / ATP Citrate Lyase (5)
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6His-SUMO, N-terminus (1)
His,N-terminus (1)
Myc-DDK (Flag) (3)
Human (4)
293T Cells (1)
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HEK 293 Cells (2)
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ACLY / ATP Citrate Lyase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
120.7 kDa
100 µg/$494
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Insect Cells
His,N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
123 kDa
50 µg/$1,007
ACLY / ATP Citrate Lyase Protein - Purified recombinant protein ACLY was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
120.7 kDa
20 µg/$1,107
ACLY / ATP Citrate Lyase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
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293T Cells
Myc-DDK (Flag)
120.7 kDa
20 µg/$150
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).