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AK1 / Adenylate Kinase 1

adenylate kinase 1

Adenylate kinase is an enzyme involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. AK1 is involved in the adenine nucleotide catabolic pathway, production of ADP, and in the homeostasis of guanine nucleotide pools. Adenylate kinase 1 phosphorylates AMP in red blood cells; deficiency in AK1 is the cause nonspherocytic hemolytic anemia. This small ubiquitous enzyme is essential for maintenance and cell growth. Three isozymes of adenylate kinase have been identified in vertebrates. AK1 is found in the cytosol of skeletal muscle, brain and erythrocytes, whereas AK2 and AK3 are found in the mitochondria of other tissues including liver and heart. Alternative splicing of the AK1 gene yields two transcripts that differ in the 3-prime non-coding region and use two distinct polyadenylation signals.

Gene Name: adenylate kinase 1
Family/Subfamily: Non-protein Kinase , not assigned-Non-protein Kinase
Synonyms: AK1, Adenylate kinase isoenzyme 1, Adenylate kinase 1, AK 1, ATP-AMP transphosphorylase 1, Myokinase
Target Sequences: NM_000476 NP_000467.1 P00568

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Proteins (7)
Over-Expression Lysate (2)
Recombinant (5)
AK1 / Adenylate Kinase 1 (7)
No (7)
GST (1)
His (1)
His,N-terminus (1)
His-SUMO (1)
Myc-DDK (Flag) (3)
Human (6)
293T Cells (1)
E. coli (3)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (2)
Biologically active (1)
AK1 / Adenylate Kinase 1 Protein
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E. coli
His
Biologically active
100 µg/$361; 500 µg/$739; 20 µg/$261
AK1 / Adenylate Kinase 1 Protein
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E. coli
His-SUMO
37.61 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
AK1 / Adenylate Kinase 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
21.5 kDa
100 µg/$494
AK1 / Adenylate Kinase 1 Protein - 12.5% SDS-PAGE of human AK1 stained with Coomassie Blue
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Wheat Germ Extract
GST
47.08 kDa
10 µg/$479; 25 µg/$670
AK1 / Adenylate Kinase 1 Protein - Purified recombinant protein AK1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
21.5 kDa
20 µg/$1,107
AK1 / Adenylate Kinase 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
21.5 kDa
20 µg/$150
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E. coli
His,N-terminus
23.5 kDa
50 µg/$561
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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).