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AOC3 / VAP-1

amine oxidase, copper containing 3

AOC3 / VAP-1 is a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants.

Gene Name: amine oxidase, copper containing 3
Synonyms: AOC3, Copper amine oxidase, HPAO, Membrane primary amine oxidase, SSAO, VAP-1, VAP1, Vascular adhesion protein 1
Target Sequences: NM_003734 NP_003725.1 Q16853

Publications (1)

1
Tripartite factors leading to molecular divergence between human and murine smooth muscle. Soo Jung Lee, Sabrina Blanchett-Anderson, Simon G Keep, Mitchell B Gasche, Michael M Wang. PloS one. 2020 January;15:e0227672. [Full Text Article] [PubMed:31945134] [PMC:PMC6964862] Related Antibodies: LS-B13865.

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Proteins (9)
Over-Expression Lysate (2)
Recombinant (7)
AOC3 / VAP-1 (9)
No (9)
6His, C-terminus (2)
Fc, C-terminus (1)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (2)
Human (8)
293T Cells (1)
CHO Cells (2)
HEK 293 Cells (1)
Human Cells (1)
Mammalian Cells (2)
Wheat Germ Extract (2)
Purified (3)
Low endotoxin level (5)
Biologically active (2)
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CHO Cells
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
2 µg/$268; 10 µg/$349
AOC3 / VAP-1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
84.4 kDa
100 µg/$494
AOC3 / VAP-1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
AOC3 / VAP-1 Protein - 12.5% SDS-PAGE of human AOC3 stained with Coomassie Blue
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Wheat Germ Extract
GST
111 kDa
2 µg/$439
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
82.6kD
10 µg/$343; 50 µg/$651; 500 µg/$2,403; 1 mg/$3,431
AOC3 / VAP-1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
84.4 kDa
20 µg/$150
Select
Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
82.6 kDa
10 µg/$429; 50 µg/$805
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Mammalian Cells
Fc, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
108.5 kDa
10 µg/$371; 50 µg/$1,177
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CHO Cells
None
Biologically active
Less than 0.1 ng/µg of protein.
The 737 amino acid recombinant protein has a predicted molecular mass of approximately 84 kD. Recombinant VAP-1 is a mixture of monomers and disulfide linked homodimers. The predicted N-terminal amino acid is Gly.
10 µg/$352
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).