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ATP6V0D1

ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1

ATP6V0D1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This encoded protein is known as the D subunit and is found ubiquitously.

Gene Name: ATPase, H+ transporting, lysosomal 38kDa, V0 subunit d1
Family/Subfamily: Transporter , ATPase - V type
Synonyms: ATP6V0D1, 32 kDa accessory protein, p39, V-ATPase, subunit D, VPATPD, V-ATPase subunit d 1, VATX, VMA6, ATP6D, ATP6DV, V-ATPase AC39 subunit, V-ATPase d subunit
Target Sequences: X71490 NP_004682.2 P61421

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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
ATP6V0D1 (6)
No (6)
GST (1)
GST, N-terminus (2)
Myc-DDK (Flag) (3)
Human (5)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (2)
ATP6V0D1 Protein - 12.5% SDS-PAGE of human ATP6V0D1 stained with Coomassie Blue
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Wheat Germ Extract
GST
64.35 kDa
10 µg/$479; 25 µg/$670
ATP6V0D1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
ATP6V0D1 Protein - Purified recombinant protein ATP6V0D1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
40.1 kDa
20 µg/$1,107
ATP6V0D1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
40.1 kDa
100 µg/$494
ATP6V0D1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
40.1 kDa
20 µg/$150
ATP6V0D1 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
GST, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).