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AUTL1 / ATG4C

autophagy related 4C, cysteine peptidase

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene Name: autophagy related 4C, cysteine peptidase
Synonyms: ATG4C, AUTL3, APG4-C, APG4C, AUTL1, Autophagin-3, APG4 autophagy 4 homolog C, Cysteine protease ATG4C
Target Sequences: NM_032852 NP_116241.2 Q96DT6

Publications (1)

1
Temporal orchestration of circadian autophagy rhythm by C/EBP. Ma D, Panda S, Lin JD. The EMBO journal. 2011 30:4642-51. [PubMed:21897364] [PMC:PMC3243590] Related Antibodies: LS-C156598.

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Products
Antibodies (54)
Primary (54)
AUTL1 / ATG4C (54)
Human (47)
Mouse (24)
Rat (14)
Bovine (3)
Chimpanzee (3)
Gibbon (3)
Horse (3)
Monkey (1)
IHC (15)
IHC-P (7)
WB (47)
ELISA (17)
ICC (1)
IF (28)
Peptide-ELISA (2)
rabbit (44)
mouse (9)
sheep (1)
Product Group
IHCPlus (1)
IgG (17)
IgG1 (1)
IgG1,k (7)
monoclonal mc (8)
polyclonal pc (46)
287CT12.2.2 (6)
2E10H7 (1)
APC Conjugated (3)
Atto 390 Conjugated (2)
Atto 488 Conjugated (2)
Atto 594 Conjugated (2)
Azide-free (1)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (3)
PE Conjugated (1)
PerCP Conjugated (2)
RPE Conjugated (2)
Unconjugated (27)
aa175-224 (4)
40-120 aa, N-terminal (1)
C-Terminus (1)
N-Terminus (1)
aa167-196 (1)
aa21-70 (1)
aa259-458 (1)
aa33-62 (1)
aa419-448 (1)
No (53)
Yes (1)
AUTL1 / ATG4C Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (aa419-448) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
AUTL1 / ATG4C Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (aa33-62) Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
AUTL1 / ATG4C Antibody - Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$460
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AUTL1 / ATG4C Sheep anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
500 µg/$393
AUTL1 / ATG4C Antibody - Immunohistochemical analysis of ATG4C staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (N-Terminus) Antibody
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100 µl/$299
AUTL1 / ATG4C Antibody - Peptide - + Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue using ATG4C antibody.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$357
AUTL1 / ATG4C Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
AUTL1 / ATG4C Antibody - Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ATG4C Antibody. The picture on the right is blocked with the synthesized peptide.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (aa21-70) Antibody
Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (40-120 aa, N-terminal) Antibody
Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, WB
Unconjugated
100 µg/$331
AUTL1 / ATG4C Antibody - Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (aa259-458) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
AUTL1 / ATG4C Antibody - Immunohistochemistry of paraffin-embedded human testis tissue using ATG4C Antibody at dilution of 1:100
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC
Unconjugated
50 µg/$294; 100 µg/$360
AUTL1 / ATG4C Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (aa167-196) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
AUTL1 / ATG4C Antibody - Western blot analysis using ATG4C mAb against HEK293 (1) and ATG4C (AA: 321-458)-hIgGFc transfected HEK293 (2) cell lysate.
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AUTL1 / ATG4C Mouse anti-Human Monoclonal (2E10H7) Antibody
Human
ELISA, WB
Unconjugated
100 µl/$459
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, WB
Unconjugated
100 µg/$442
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AUTL1 / ATG4C Mouse anti-Mouse Monoclonal (287CT12.2.2) Antibody
Mouse
ELISA, WB
Unconjugated
200 µl/$734
AUTL1 / ATG4C Antibody - Western blot of testis cell lysate, using ATG4C antibody.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$356; 100 µl/$461
AUTL1 / ATG4C Antibody - Western blot analysis of extracts of various cell lines.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Human
IHC, WB
Unconjugated
20 µl/$275; 100 µl/$389
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4C Antibody. (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal Antibody
Human
IF, WB
Unconjugated
100 µg/$442
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (FITC) Antibody
Mouse, Rat, Human
IF, WB
FITC Conjugated
100 µg/$473
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (RPE) Antibody
Mouse, Rat, Human
IF, WB
RPE Conjugated
100 µg/$477
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (APC) Antibody
Mouse, Rat, Human
IF, WB
APC Conjugated
100 µg/$479
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (PerCP) Antibody
Mouse, Rat, Human
IF, WB
PerCP Conjugated
100 µg/$479
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (Atto 390) Antibody
Mouse, Rat, Human
IF, WB
Atto 390 Conjugated
100 µg/$480
AUTL1 / ATG4C Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
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AUTL1 / ATG4C Rabbit anti-Human Polyclonal (Atto 488) Antibody
Mouse, Rat, Human
IF, WB
Atto 488 Conjugated
100 µg/$479
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).