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AUTL1 / ATG4C

autophagy related 4C, cysteine peptidase

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene Name: autophagy related 4C, cysteine peptidase
Synonyms: ATG4C, AUTL3, APG4-C, APG4C, AUTL1, Autophagin-3, APG4 autophagy 4 homolog C, Cysteine protease ATG4C
Target Sequences: NM_032852 NP_116241.2 Q96DT6

Publications (1)

1
Temporal orchestration of circadian autophagy rhythm by C/EBP. Ma D, Panda S, Lin JD. The EMBO journal. 2011 30:4642-51. [PubMed:21897364] [PMC:PMC3243590] Related Antibodies: LS-C156598.

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Products
Proteins (9)
Over-Expression Lysate (4)
Recombinant (5)
AUTL1 / ATG4C (9)
No (9)
6His, C-terminus (2)
GST (1)
Myc-DDK (Flag) (6)
Human (7)
293T Cells (2)
E. coli (2)
HEK 293 Cells (4)
Wheat Germ Extract (1)
Purified (2)
Low endotoxin level (2)
AUTL1 / ATG4C Protein - 12.5% SDS-PAGE of human ATG4C stained with Coomassie Blue
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Wheat Germ Extract
GST
78.9 kDa
10 µg/$479; 25 µg/$670
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E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
53.56kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
AUTL1 / ATG4C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
52.3 kDa
20 µg/$150
AUTL1 / ATG4C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
52.3 kDa
20 µg/$150
AUTL1 / ATG4C Protein - Purified recombinant protein ATG4C was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
52.3 kDa
20 µg/$1,107
AUTL1 / ATG4C Protein - Purified recombinant protein ATG4C was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
52.3 kDa
20 µg/$1,107
AUTL1 / ATG4C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
52.3 kDa
100 µg/$494
AUTL1 / ATG4C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
52.3 kDa
100 µg/$494
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
53.6 kDa
10 µg/$344; 50 µg/$584
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).