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CMA1 / Mast Cell Chymase

chymase 1, mast cell

CMA1 / MCT1 / Chymase is a chymotryptic serine proteinase that belongs to the peptidase family S1. It is expressed in mast cells and is thought to function in the degradation of the extracellular matrix, the regulation of submucosal gland secretion, and the generation of vasoactive peptides. In the heart and blood vessels, this protein, rather than angiotensin converting enzyme, is largely responsible for converting angiotensin I to the vasoactive peptide angiotensin II. Alternative splicing results in multiple variants.

Gene Name: chymase 1, mast cell
Family/Subfamily: Protease , Serine S1
Synonyms: CMA1, Chymase, Chymase, heart, Chymase, mast cell, CYH, Mast cell chymase, Mcpt5, MCT1, Mast cell protease 5, Mast cell protease I, Heart chymase, Alpha-chymase, Chymase 1, Chymase 1, mast cell, CYM
Target Sequences: NM_001836 NP_001827.1 P23946

Publications (1)

1
The muscular expression of RAS in patients with achalasia. Casselbrant A, Kostic S, Lönroth H. Journal of the renin-angiotensin-aldosterone system : JRAAS. 2014 16:578-86. (WB; Human) [Full Text Article] [PubMed:24468662]

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Proteins (12)
Over-Expression Lysate (2)
Recombinant (10)
CMA1 / Mast Cell Chymase (12)
No (12)
6His, N-terminus (2)
6His-SUMO, N-terminus (1)
His (2)
His, N-Terminal (2)
His-GST (1)
His-SUMO (1)
His-T7 (1)
Myc-DDK (Flag) (2)
Crab-eating macaque (1)
Dog (2)
Human (5)
Mouse (2)
Rat (1)
293T Cells (1)
Baculovirus (1)
E. coli (7)
HEK 293 Cells (2)
Yeast (1)
Purified (4)
Low endotoxin level (1)
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E. coli
His-SUMO
41.4 kD
1 mg/$1,679; 100 µg/$598; 20 µg/$405
CMA1 / Mast Cell Chymase Protein
Select
E. coli
6His, N-terminus
29.3 kD
1 mg/$1,679; 100 µg/$500; 20 µg/$364
CMA1 / Mast Cell Chymase Protein - Recombinant Chymase 1, Mast Cell (CMA1) by SDS-PAGE
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E. coli
His, N-Terminal
29kDa
10 µg/$281; 50 µg/$440; 200 µg/$877; 100 µg/$702; 1 mg/$1,983; 500 µg/$1,523; 5 mg/$3,067; 2 mg/$2,170
Select
Yeast
His
27.4 kD
100 µg/$679; 20 µg/$429; 1 mg/$1,973
CMA1 / Mast Cell Chymase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
27.1 kDa
20 µg/$150
CMA1 / Mast Cell Chymase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
27.1 kDa
100 µg/$494
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Baculovirus
His
Less than 1.0 EU/µg protein (determined by LAL method).
26.6 kDa
50 µg/$854
CMA1 / Mast Cell Chymase Protein - Recombinant  Chymase 1, Mast Cell By SDS-PAGE
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E. coli
His-GST
58.6 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
CMA1 / Mast Cell Chymase Protein - Recombinant Chymase 1, Mast Cell By SDS-PAGE
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E. coli
His-T7
29.2 kDa
10 µg/$286; 50 µg/$472; 100 µg/$752; 200 µg/$942; 1 mg/$2,069; 500 µg/$1,600; 5 mg/$3,291; 2 mg/$2,257
CMA1 / Mast Cell Chymase Protein
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HEK 293 Cells
His, N-Terminal
25.9KDa
20 µg/$1,107
CMA1 / Mast Cell Chymase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
CMA1 / Mast Cell Chymase Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$598; 20 µg/$405
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).