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DLL1

delta-like 1 (Drosophila)

Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.

Gene Name: delta-like 1 (Drosophila)
Synonyms: DLL1, Delta-like 1 (Drosophila), Delta (Drosophila)-like 1, Drosophila Delta homolog 1, Delta-like protein 1, Delta1, H-Delta-1, DL1, Npa
Target Sequences: NM_005618 NP_005609.3 O00548

Publications (5)

1
Differential expression of Notch receptors and their ligands in desmoplastic ameloblastoma. Siar CH, Nakano K, Han PP, Nagatsuka H, Ng KH, Kawakami T. Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology. 2010 39:552-8. (IHC-P; Human) [PubMed:20337864] Related Antibodies: LS-B72.
2
Notch4 overexpression in ameloblastoma correlates with the solid/multicystic phenotype. Siar CH, Nagatsuka H, Chuah KS, Rivera RS, Nakano K, Ng KH, Kawakami T. Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics. 2010 110:224-33. (IHC-P; Human) [PubMed:20659700] Related Antibodies: LS-B72.
3
Immunolocalization of notch signaling protein molecules in a maxillary chondrosarcoma and its recurrent tumor. Siar CH, Ha KO, Aung LO, Nakano K, Tsujigiwa H, Nagatsuka H, Ng KH, Kawakami T. European journal of medical research. 2010 15:456-60. (IHC-P; Human) [Full Text Article] [PubMed:21156405] [PMC:PMC3352190] Related Antibodies: LS-B72.
4
Notch signaling and ghost cell fate in the calcifying cystic odontogenic tumor. Siar CH, Kawakami T, Buery RR, Nakano K, Tomida M, Tsujigiwa H, Han PP, Nagatsuka H, Ng KH. European journal of medical research. 2011 16:501-6. (IHC-P; Human) [PubMed:22027644] [PMC:PMC3351808] Related Antibodies: LS-B72.
5
Age-related properties of the tumour vasculature in renal cell carcinoma. Meehan B, Appu S, St Croix B, Rak-Poznanska K, Klotz L, Rak J. BJU international. 2011 February;107:416-24. (Mouse) [Full Text Article] [PubMed:20804487] Related Antibodies: LS-B72.
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Products
Proteins (16)
Over-Expression Lysate (2)
Recombinant (14)
DLL1 (16)
Bind (2)
ELISA (2)
Func (2)
SDS-PAGE (2)
No (16)
DDK (Flag) (1)
Fc, C-terminus (1)
GST, N-terminus (1)
His (3)
His, C-Terminal (1)
His,C-terminus (3)
Human IgG1 Fc (1)
Human IgG1 Fc, C-terminus (1)
Myc-DDK (Flag) (3)
Human (12)
Mouse (3)
293T Cells (1)
CHO Cells (1)
E. coli (1)
HEK 293 Cells (7)
Human (2)
Human Cells (2)
Mammalian Cells (1)
Wheat Germ Extract (1)
Purified (5)
Low endotoxin level (12)
Biologically active (3)
DLL1 Protein - Adipogenesis inhibition of 3T3L1 cells.3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM dexamethasone, 0.5mM IBMX, 10 ug/ml insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml hDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Select
CHO Cells
Human IgG1 Fc
Less than 0.01 EU/µg protein (determined by LAL method).
~100kDa (SDS-PAGE)
50 µg/$798; 10 µg/$389
Select
HEK 293 Cells
Human IgG1 Fc, C-terminus
Less than 0.1 EU/µg protein (determined by LAL method).
~100 kDa (SDS-PAGE)
10 µg/$316; 50 µg/$428
Select
HEK 293 Cells
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
57.0-60.0 kDa
5 µg/$268; 25 µg/$349
DLL1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
DLL1 Protein - Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 µg/mL.
Select
HEK 293 Cells
His,C-terminus
Bind ELISA Func SDS-PAGE
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant human DLL1 consists of 530 amino acids and predictes a molecular mass of 57.4 kDa. In SDS-PAGE under reducing conditions, rh DLL1 migrates as an approximately 58 kDa band due to glycosylation.
100 µg/$441; 1 mg/$2,003
DLL1 Protein
Select
HEK 293 Cells
His,C-terminus
Bind ELISA Func SDS-PAGE
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant mouse DLL1 consists of 506 amino acids and has a predicted molecular mass of 55 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm DLL1 is approximately 65 kDa due to glycosylation.
100 µg/$441; 1 mg/$2,003
DLL1 Protein - Purified recombinant protein DLL1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
77.9 kDa
20 µg/$1,107
Select
Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
57.4 kDa
100 µg/$721
DLL1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
77.9 kDa
100 µg/$710
DLL1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
77.9 kDa
20 µg/$215
DLL1 Protein - Recombinant Human Delta-like Protein 1/DLL1 (C-Fc)
Select
Human
Fc, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
83.3 kDa
10 µg/$265; 50 µg/$373
Select
Mammalian Cells
His,C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
57.3 kDa
10 µg/$277; 50 µg/$389
DLL1 Protein - Adipogenesis inhibition of MSCs. MSCs (Mesenchymal stem cells) were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin and glutamine. For differentiation of MSCs, MSCs were cultured in adipogenic medium which was growth medium supplemented with 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin, 100 uM Indomethacin (day 1). Medium was changed every 3 days. Staining with Oil Red O was typically performed on day 30. For negative controls, TNF-alpha (20ng/ml) was added. To immobilize Notch ligands on the plastic surface of the culture plates, plates were first incubated with a solution of anti-FLAG antibody (10 ug/ml) in PBS for 30 minutes at 37 degrees C. Coated plates were blocked with growth medium for at least 30 minutess, incubated with solution of DLL1 (human) (rec.) (5 ug/ml) or mCD137L-FLAG (5 ug/ml) in growth medium for 2 hours at 37 degrees C. Plates were then used to differentiate MSCs.
Select
HEK 293 Cells
DDK (Flag)
Less than 0.1 EU/µg protein (determined by LAL method).
~70kDa (SDS-PAGE)
50 µg/$431; 10 µg/$323
Select
E. coli
214 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
23.65 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
Select
Human
His, C-Terminal
Less than 1.0 EU/µg protein (determined by LAL method).
57.3kD
50 µg/$395; 500 µg/$1,585; 1 mg/$2,157; 10 µg/$277
Select
Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
55 kDa
100 µg/$721
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).