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EGF

epidermal growth factor

EGF is a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants.

Gene Name: epidermal growth factor
Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor
Target Sequences: NM_001963 NP_001954.2 P01133

Publications (1)

1
The Histopathological Investigation of Red and Blue Light Emitting Diode on Treating Skin Wounds in Japanese Big-Ear White Rabbit. Li Y, Zhang J, Xu Y, Han Y, Jiang B, Huang L, Zhu H, Xu Y, Yang W, Qin C. PloS one. 2016 11:e0157898. (WB; Human) [Full Text Article] [PubMed:27347879] [PMC:PMC4922561] Related Antibodies: LS-B11905.

☰ Filters
Products
Proteins (50)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (3)
Recombinant (45)
EGF (50)
SDS-PAGE (1)
Animal-free (2)
No (50)
6His, C-terminus (3)
6His, N-terminus (1)
8His, N-terminus (1)
Fc, N-terminus (1)
GST (1)
GST, N-terminus (1)
His (5)
His, N-Terminal (2)
His,C-terminus (1)
His-GST (2)
His-S (1)
His-SUMO, N-terminus (1)
Human IgG1 Fc (1)
Myc-DDK (Flag) (3)
Dog (3)
Human (27)
Mouse (11)
Pig (2)
Rat (6)
293T Cells (1)
CHO Cells (3)
E. coli (38)
HEK 293 Cells (6)
Wheat Germ Extract (1)
Purified (15)
Low endotoxin level (26)
Biologically active (23)
Animal-free (2)
EGF Protein - Active Epidermal Growth Factor (EGF) by WB
Select
E. coli
His, N-Terminal
18kDa
10 µg/$262; 50 µg/$339; 200 µg/$642; 100 µg/$512; 1 mg/$1,623; 500 µg/$1,214; 5 mg/$2,562; 2 mg/$1,796
Select
E. coli
None
Biologically active
6.2 kDa
100 µg/$270; 500 µg/$349
EGF Protein - Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 ng/mL.
Select
E. coli
None
SDS-PAGE
Biologically active
The recombinant human EGF consisting of 54 amino acids and has a calculated molecular mass of 6.3 kDa as estimated in SDS-PAGE under reducing conditions.
1 mg/$372; 100 µg/$255
EGF Protein - Recombinant Epidermal Growth Factor (EGF) by SDS-PAGE
Select
E. coli
His, N-Terminal
38kDa
10 µg/$275; 50 µg/$402; 200 µg/$802; 100 µg/$642; 1 mg/$1,876; 500 µg/$1,430; 5 mg/$2,838; 2 mg/$2,060
EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Select
E. coli
None
Biologically active
6 kDa
1 mg/$469
EGF Protein - Inhibition of A431 cell proliferation by mouse EGF.
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg (determined by LAL method).
The 53 amino acid recombinant protein has a predicted molecular mass of approximately 6 kD. The DTT-reduced protein migrates at approximately 6 kD and non-reduced protein migrates at approximately 13 kD by SDS-PAGE. The N-terminal amino acid is Asparagine
100 µg/$268; 500 µg/$352
EGF Protein - Inhibition of A431 cell proliferation by human EGF.
Select
E. coli
None
Less than 0.01 ng/µg (determined by LAL method).
The 54 amino acid recombinant protein has a predicted molecular mass of approximately 6 kD. The DTT-reduced protein migrates at approximately 6 kD and non-reduced protein migrates at approximately 13 kD by SDS-PAGE. The N-terminal amino acid is Methionine
100 µg/$268; 500 µg/$352
Select
E. coli
None
6.3 kDa
100 µg/$287; 1 mg/$463
Select
E. coli
None
Less than 0.1 ng/µg protein.
6.2 kDa
100 µg/$339; 500 µg/$429; 1000 µg/$534
EGF Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
133.95 kDa
100 µg/$494
EGF Protein - Purified recombinant protein EGF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Select
HEK 293 Cells
Myc-DDK (Flag)
131.6 kDa
20 µg/$1,107
Select
None
Biologically active
6.2 kD
0.5 mg/$484
EGF Protein - 12.5% SDS-PAGE Stained with Coomassie Blue
Select
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
EGF Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
133.95 kDa
20 µg/$150
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
6.2 kDa
20 µg/$268; 100 µg/$349
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
20 µg/$282; 100 µg/$370
Select
E. coli
None
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
6.0 kDa
100 µg/$268; 500 µg/$349
Select
E. coli
None
Biologically active
Less than 0.01 ng/µg of protein (Less than 0.1 EU/µg).
100 µg/$282; 500 µg/$365
Select
E. coli
GST
33 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
Select
HEK 293 Cells
6His, N-terminus
7.7 kDa
200 µl/$283
EGF Protein - Biological Activity EGF, Human stimulates cell proliferation of BALB/c 3T3 cells. The ED 50 for this effect is typically 5–100pg/mL.
Select
E. coli
None
Biologically active
Less than 0.2 EU/µg protein (determined by LAL method).
6.2 kDa (SDS-PAGE)
50 µg/$304; 10 µg/$246; 1 mg/$381
Select
E. coli
None
Biologically active
Less than 0.2 EU/µg protein (determined by LAL method).
6.2 kDa (SDS-PAGE)
50 µg/$262; 10 µg/$245; 1 mg/$432
Select
HEK 293 Cells
8His, N-terminus
7.7 kDa
100 µg/$298
Select
CHO Cells
None
Biologically active
Less than 0.2 EU/µg protein (determined by LAL method).
~6 kDa (SDS-PAGE)
50 µg/$278; 10 µg/$246; 1 mg/$844
Select
CHO Cells
Human IgG1 Fc
Less than 0.06 EU/µg protein (determined by LAL method).
100 µg/$351
Viewing 1-25 of 50 product results


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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).