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GFP
Green Fluorescent Protein (GFP)
Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation.
Publications (32)
1
Direct interaction between the actin-binding protein filamin-A and the inwardly rectifying potassium channel, Kir2.1. Sampson LJ, Leyland ML, Dart C. The Journal of biological chemistry. 2003 278:41988-97.
[PubMed:12923176]
2
The Vif protein of HIV triggers degradation of the human antiretroviral DNA deaminase APOBEC3G. Conticello SG, Harris RS, Neuberger MS. Current biology : CB. 2003 13:2009-13.
[PubMed:14614829]
3
Molecular cloning, characterization and tissue expression of porcine Toll-like receptor 4. Alvarez B, Revilla C, Chamorro S, Lpez-Fraga M, Alonso F, Domnguez J, Ezquerra A. Developmental and comparative immunology. 2006 30:345-55.
[PubMed:16139355]
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![GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with monoclonal anti-GFP antibody. GFP recombinant protein was expressed in HeLa cells, where 50 ng (lane 1), 100 ng (lane 2) and 500 ng (lane 3) of lysate were loaded per lane. Mab anti-GFP detects a 27 kD band corresponding to the epitope tag GFP. The cell lysates were prepared in a RIPA buffer containing 200 mM NaCl. A 4-12% Bis-Tris gradient gel (Invitrogen) was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed with the primary antibody diluted to 1.0 mg/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Goat-a-Mouse IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/gfp-antibody-clone-9f9.f9-ls-c154208/81891_5149892.jpg)
GFP Mouse anti-Aequorea victoria Monoclonal (9F9.F9) Antibody
Aequorea victoria
ELISA, Flo, IF, IHC, WB
Unconjugated
1 mg/$921
![GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP protein detected with polyclonal anti-GFP antibody. Wild type GFP (0.1 ug) was used to spike 30 ug of a HeLa whole cell lysate. This antibody detects a 27 kD band corresponding to the epitope tag GFP. A 4-20% Tris-Glycine gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking with 5% BLOTTO in PBS, the membrane was probed overnight at 4C with the primary antibody diluted in 5% BLOTTO to 1:1000, followed by washes and reaction with a 1:10000 dilution of IRDye 800 conjugated Goat-a-Rabbit IgG [H&L] MX10 (. IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.](https://lsbio-7d62.kxcdn.com/image2/gfp-antibody-ls-c154219/81899_5149904.jpg)
GFP Rabbit anti-Aequorea victoria Polyclonal Antibody
Aequorea victoria
ELISA, IF, IHC, WB
Unconjugated
100 µg/$465
GFP Goat anti-Aequorea victoria Polyclonal (HRP) Antibody
Aequorea victoria
ELISA, IF, IHC, WB
HRP Conjugated
500 µg/$743
GFP Goat anti-Aequorea victoria Polyclonal Antibody
Aequorea victoria
ELISA, IHC, IHC-P, IP, WB
Unconjugated
100 µg/$762; 500 µg/$960
GFP Goat anti-Aequorea victoria Polyclonal Antibody
Aequorea victoria
ELISA, IF, IHC, IP, WB
Unconjugated
1 mg/$715
GFP Mouse Monoclonal (N/K) Antibody
Aequorea victoria
ELISA, IHC-Fr, IHC-P, WB
Unconjugated
0.1 ml/$662
GFP Rabbit anti-Aequorea victoria Polyclonal (Polyclonal) Antibody
All species
ICC, IP, WB
Unconjugated
0.1 mg/$316
GFP Goat anti-Aequorea victoria Polyclonal (Biotin) Antibody
Aequorea victoria
ELISA, IHC, WB
Biotin Conjugated
500 µg/$743
![GFP Antibody - Anti-GFP Antibody - Western Blot. Western blot of GFP recombinant protein detected with polyclonal anti-GFP antibody. Lane 1 shows detection of a 33 kD band corresponding to a GFP containing recombinant protein (arrowhead) expressed in HeLa cells. Lane 2 shows no staining of a mock transfected HeLa cell lysate. A 4-12% Bis-Tris gradient gel was used for SDS-PAGE. The protein was transferred to nitrocellulose using standard methods. After blocking the membrane was probed with the primary antibody diluted to 1 ug/ml for 1 h at room temperature followed by washes and reaction with a 1:2500 dilution of IRDye 800 conjugated Donkey-a-Goat IgG [H&L] MX7 (. The IRDye 800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. This image was taken for the unconjugated form of this product. Other forms have not been tested.](https://lsbio-7d62.kxcdn.com/image2/gfp-antibody-fitc-ls-c154186/81887_5149889.jpg)
GFP Goat anti-Aequorea victoria Polyclonal (FITC) Antibody
Aequorea victoria
FLISA, IF, WB
FITC Conjugated
1 mg/$768
GFP Mouse anti-Aequorea victoria Monoclonal Antibody
Aequorea victoria
ELISA, IHC, IHC-Fr, IHC-P, WB
Unconjugated
100 µl/$913
Sandwich
96-Well Strip Plate
All species
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Biological Fluids
1 Plate/$831
Sandwich
96-Well Strip Plate
All species
15.6 - 1000 pg/ml
Colorimetric - 450nm (TMB)
Cell Lysates
1 Plate/$632
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$345; 500 µg/$657; 20 µg/$254
GFP Mouse anti-Mouse Monoclonal (1E4) Antibody
ICC, IHC, IP, WB
Unconjugated
100 µg/$400
GFP Rabbit anti-Aequorea victoria Polyclonal (Biotin) Antibody
Aequorea victoria
ELISA, IF, IHC, WB
Biotin Conjugated
100 µg/$544
GFP Chicken anti-Aequorea victoria Polyclonal Antibody
Aequorea victoria
ELISA, IF, IHC, WB
Unconjugated
100 µg/$462
GFP Rabbit anti-Aequorea victoria Polyclonal Antibody
Aequorea victoria
ICC, IF, IHC, IHC-P, IP, WB
Unconjugated
50 µl/$771
Development Kit
Antibody Pair and Standard
All species
3.12 - 200 ng/ml
Colorimetric - 450nm (TMB)
Biological Fluids
500 Wells/$1,432; 1000 Wells/$2,163
GFP Mouse anti-Aequorea victoria Monoclonal (HRP) (9F9.F9) Antibody
Aequorea victoria
ELISA, IHC, WB
HRP Conjugated
1 mg/$947
GFP Mouse anti-Aequorea victoria Monoclonal (Biotin) (9F9.F9) Antibody
Aequorea victoria
ELISA, IHC, WB
Biotin Conjugated
1 mg/$947
GFP Mouse anti-Aequorea victoria Monoclonal (FITC) Antibody
Aequorea victoria
Flo, IF
FITC Conjugated
1 mg/$1,355
GFP Goat anti-Aequorea victoria Polyclonal (HRP) Antibody
Aequorea victoria
ELISA, IHC, WB
HRP Conjugated
1 mg/$749
GFP Rabbit anti-Aequorea victoria Polyclonal (aa204-217) Antibody
Aequorea victoria
WB
Unconjugated
100 µg/$634
GFP Rabbit Polyclonal (Biotin) Antibody
All species, Human
WB
Biotin Conjugated
100 µl/$321; 200 µl/$368; 500 µl/$526; 1 ml/$608; 2 ml/$839; 5 ml/$1,379
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