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GFP

Green Fluorescent Protein (GFP)

Green Fluorescent Protein (GFP) exhibits bright green fluorescence when exposed to blue light. Although many other marine organisms have similar green fluorescent proteins, GFP traditionally refers to the protein first isolated from the jellyfish Aequorea victoria. GFP from A. victoria has a major excitation peak at a wavelength of 395 nm and a minor one at 475 nm. Its emission peak is at 509 nm, which is in the lower green portion of the visible spectrum. GFP from the sea pansy (Renilla reniformis) has a single major excitation peak at 498 nm. In cell and molecular biology, the GFP gene is frequently used as a reporter of expression. In modified forms it has been used to make biosensors, and many animals have been created that express GFP as a proof-of-concept that a gene can be expressed throughout a given organism. The GFP gene can be introduced into organisms and maintained in their genome through breeding, injection with a viral vector, or cell transformation.

Gene Name: Green Fluorescent Protein (GFP)
Synonyms: GFP
Target Sequences: M62653 P42212 P42212

Publications (32)

1
Direct interaction between the actin-binding protein filamin-A and the inwardly rectifying potassium channel, Kir2.1. Sampson LJ, Leyland ML, Dart C. The Journal of biological chemistry. 2003 278:41988-97. [PubMed:12923176]
2
The Vif protein of HIV triggers degradation of the human antiretroviral DNA deaminase APOBEC3G. Conticello SG, Harris RS, Neuberger MS. Current biology : CB. 2003 13:2009-13. [PubMed:14614829]
3
Molecular cloning, characterization and tissue expression of porcine Toll-like receptor 4. Alvarez B, Revilla C, Chamorro S, Lpez-Fraga M, Alonso F, Domnguez J, Ezquerra A. Developmental and comparative immunology. 2006 30:345-55. [PubMed:16139355]
4
Type II epithelial cells are critical target for hyperoxia-mediated impairment of postnatal lung development. Yee M, Vitiello PF, Roper JM, Staversky RJ, Wright TW, McGrath-Morrow SA, Maniscalco WM, Finkelstein JN, O'Reilly MA. American journal of physiology. Lung cellular and molecular physiology. 2006 291:L1101-11. [PubMed:16861382]
5
Pseudomonas aeruginosa type III-secreted toxin ExoT inhibits host-cell division by targeting cytokinesis at multiple steps. Shafikhani SH, Engel J. Proceedings of the National Academy of Sciences of the United States of America. 2006 103:15605-10. [PubMed:17030800] [PMC:PMC1622869]
6
ORF73-null murine gammaherpesvirus 68 reveals roles for mLANA and p53 in virus replication. Forrest JC, Paden CR, Allen RD, Collins J, Speck SH. Journal of virology. 2007 81:11957-71. [PubMed:17699571] [PMC:PMC2168792] Related Antibodies: LS-C154208.
7
Identification of a novel mitotic phosphorylation motif associated with protein localization to the mitotic apparatus. Yang F, Camp DG, Gritsenko MA, Luo Q, Kelly RT, Clauss TR, Brinkley WR, Smith RD, Stenoien DL. Journal of cell science. 2007 120:4060-70. [PubMed:17971412] Related Antibodies: LS-C154208.
8
B-cell receptor activation inhibits AID expression through calmodulin inhibition of E-proteins. Hauser J, Sveshnikova N, Wallenius A, Baradaran S, Saarikettu J, Grundstrm T. Proceedings of the National Academy of Sciences of the United States of America. 2008 105:1267-72. [PubMed:18203819] [PMC:PMC2234127]
9
Aire controls the differentiation program of thymic epithelial cells in the medulla for the establishment of self-tolerance. Yano M, Kuroda N, Han H, Meguro-Horike M, Nishikawa Y, Kiyonari H, Maemura K, Yanagawa Y, Obata K, Takahashi S, Ikawa T, Satoh R, Kawamoto H, Mouri Y, Matsumoto M. The Journal of experimental medicine. 2008 205:2827-38. [PubMed:19015306] [PMC:PMC2585853]
10
Type A GABA-receptor-dependent synaptic transmission sculpts dendritic arbor structure in Xenopus tadpoles in vivo. Shen W, Da Silva JS, He H, Cline HT. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2009 29:5032-43. [PubMed:19369572] [PMC:PMC2706946] Related Antibodies: LS-C154208.
11
Stabilizers of the Max homodimer identified in virtual ligand screening inhibit Myc function. Jiang H, Bower KE, Beuscher AE, Zhou B, Bobkov AA, Olson AJ, Vogt PK. Molecular pharmacology. 2009 76:491-502. [PubMed:19498040] [PMC:PMC2730382]
12
ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays. Zangar RC, Daly DS, White AM, Servoss SL, Tan RM, Collett JR. Journal of proteome research. 2009 8:3937-43. [PubMed:19618941] Related Antibodies: LS-C154288.
13
Lef-1 isoforms regulate different target genes and reduce cellular adhesion. Jesse S, Koenig A, Ellenrieder V, Menke A. International journal of cancer. Journal international du cancer. 2010 126:1109-20. [PubMed:19653274] Related Antibodies: LS-C154208.
14
Splice variants of the dual specificity tyrosine phosphorylation-regulated kinase 4 (DYRK4) differ in their subcellular localization and catalytic activity. Papadopoulos C, Arato K, Lilienthal E, Zerweck J, Schutkowski M, Chatain N, Mller-Newen G, Becker W, de la Luna S. The Journal of biological chemistry. 2011 286:5494-505. [PubMed:21127067] [PMC:PMC3037663] Related Antibodies: LS-C154219.
15
The syntaxin 4 N terminus regulates its basolateral targeting by munc18c-dependent and -independent mechanisms. Torres J, Funk HM, Zegers MM, ter Beest MB. The Journal of biological chemistry. 2011 286:10834-46. [PubMed:21278252] [PMC:PMC3060534] Related Antibodies: LS-C154219.
16
Vaccinia extracellular virions enter cells by macropinocytosis and acid-activated membrane rupture. Schmidt FI, Bleck CK, Helenius A, Mercer J. The EMBO journal. 2011 30:3647-61. [PubMed:21792173] [PMC:PMC3181475] Related Antibodies: LS-C154219.
17
The role of PSD-95 and cypin in morphological changes in dendrites following sublethal NMDA exposure. Tseng CY, Firestein BL. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2011 31:15468-80. [PubMed:22031893] Related Antibodies: LS-C154312.
18
Therapeutic gene delivery and transfection in human pancreatic cancer cells using epidermal growth factor receptor-targeted gelatin nanoparticles. Xu J, Amiji M. Journal of visualized experiments : JoVE. 2012 e3612. [PubMed:22231028] [PMC:PMC3400379]
19
Multi-compartmental nanoparticles-in-emulsion formulation for macrophage-specific anti-inflammatory gene delivery. Attarwala H, Amiji M. Pharmaceutical research. 2012 29:1637-49. (ELISA; Mouse) [PubMed:22281760]
20
Retinoblastoma tumor-suppressor protein phosphorylation and inactivation depend on direct interaction with Pin1. Rizzolio F, Lucchetti C, Caligiuri I, Marchesi I, Caputo M, Klein-Szanto AJ, Bagella L, Castronovo M, Giordano A. Cell death and differentiation. 2012 19:1152-61. [PubMed:22322860] [PMC:PMC3374078] Related Antibodies: LS-C154219.
21
Tuftsin-modified alginate nanoparticles as a noncondensing macrophage-targeted DNA delivery system. Jain S, Amiji M. Biomacromolecules. 2012 13:1074-85. (ELISA; Mouse) [PubMed:22385328]
22
High-throughput, multiplexed analysis of 3-nitrotyrosine in individual proteins. Jin H, Zangar RC. Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]. 2012 Unit 17.15. [PubMed:22511115] Related Antibodies: LS-C154219.
23
CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry. Lai CY, Schwartz BE, Hsu MY. Cancer research. 2012 72:5111-8. (ICC, IHC; Human) [PubMed:22865455] [PMC:PMC3463654]
24
Regulation of postnatal forebrain amoeboid microglial cell proliferation and development by the transcription factor Runx1. Zusso M, Methot L, Lo R, Greenhalgh AD, David S, Stifani S. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2012 32:11285-98. (ICC; Mouse) [PubMed:22895712]
25
Identification of the Plasmodium berghei resistance locus 9 linked to survival on chromosome 9. Bopp SE, Rodrigo E, González-Páez GE, Frazer M, Barnes SW, Valim C, Watson J, Walker JR, Schmedt C, Winzeler EA. Malaria journal. 2013 12:316. (IHC; Mouse) [Full Text Article] [PubMed:24025732] [PMC:PMC3848760] Related Antibodies: LS-C67081.
26
Dystroglycan controls dendritic morphogenesis of hippocampal neurons in vitro. Bijata M, Wlodarczyk J, Figiel I. Frontiers in cellular neuroscience. 2015 9:199. (IHC; Aequorea victoria) [Full Text Article] [PubMed:26074769] [PMC:PMC4443029] Related Antibodies: LS-C50850.
27
Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis. Lee H, Noh H, Mun J, Gu C, Sever S, Park S. Nature communications. 2016 7:12799. (IF; Human) [Full Text Article] [PubMed:27619642] [PMC:PMC5027278]
28
Hepatocellular Carcinoma Cell-Secreted Exosomal MicroRNA-210 Promotes Angiogenesis In Vitro and In Vivo. Lin XJ, Fang JH, Yang XJ, Zhang C, Yuan Y, Zheng L, Zhuang SM. Molecular therapy. Nucleic acids. 2018 June;11:243-252. (Aequorea victoria) [Full Text Article] [PubMed:29858059] [PMC:PMC5992447] Related Antibodies: LS-C154219.
29
The nicotinic a6 subunit gene determines variability in chronic pain sensitivity via cross-inhibition of P2X2/3 receptors. Wieskopf JS, Mathur J, Limapichat W, Post MR, Al-Qazzaz M, Sorge RE, Martin LJ, Zaykin DV, Smith SB, Freitas K, Austin JS, Dai F, Zhang J, Marcovitz J, Tuttle AH, Slepian PM, Clarke S, Drenan RM, Janes J, Al Sharari S, Segall SK, Aasvang EK, Lai W, Bittner R, Richards CI, Slade GD, Kehlet H, Walker J, Maskos U, Changeux JP, Devor M, Maixner W, Diatchenko L, Belfer I, Dougherty DA, Su AI, Lummis SC, Imad Damaj M, Lester HA, Patapoutian A, Mogil JS. Science translational medicine. 2015 May;7:287. (Aequorea victoria) [Full Text Article] [PubMed:25972004] [PMC:PMC5018401]
30
Fibrotic microenvironment promotes the metastatic seeding of tumor cells via activating the fibronectin 1/secreted phosphoprotein 1-integrin signaling. Zhang C, Wu M, Zhang L, Shang LR, Fang JH, Zhuang SM. Oncotarget. 2016 July;7:45702-45714. (Aequorea victoria) [Full Text Article] [PubMed:27329720] [PMC:PMC5216754] Related Antibodies: LS-C154219.
31
Detection of membrane protein-protein interaction in planta based on dual-intein-coupled tripartite split-GFP association. Tzu-Yin Liu, Wen-Chun Chou, Wei-Yuan Chen, Ching-Yi Chu, Chen-Yi Dai, Pei-Yu Wu. The Plant journal : for cell and molecular biology. 2018 May;94:426-438. [Full Text Article] [PubMed:29451720]
32
DHHC7-mediated palmitoylation of the accessory protein barttin critically regulates the functions of ClC-K chloride channels. Nataliya Gorinski, Daniel Wojciechowski, Daria Guseva, Dalia Abdel Galil, Franziska E Mueller, Alexander Wirth, Stefan Thiemann, Andre Zeug, Silke Schmidt, Monika Zareba-Koziol, Jakub Wlodarczyk, Boris V Skryabin, Silke Glage, Martin Fischer, Samer Al-Samir, Nicole Kerkenberg, Christa Hohoff, Weiqi Zhang, Volker Endeward, Evgeni Ponimaskin. The Journal of biological chemistry. 2020 May;295:5970-5983. [Full Text Article] [PubMed:32184353] [PMC:PMC7196637] Related Antibodies: LS-C50850.
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).