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Vector Laboratories, Inc.
6737 Mowry Ave
Newark, CA 94560
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MIF

macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity.

Gene Name: macrophage migration inhibitory factor (glycosylation-inhibiting factor)
Synonyms: MIF, GLIF, L-dopachrome tautomerase, L-dopachrome isomerase, MMIF, Phenylpyruvate tautomerase
Target Sequences: NM_002415 NP_002406.1 P14174

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Products
Proteins (25)
Over-Expression Lysate (2)
Recombinant (22)
MIF (25)
No (25)
6His, C-terminus (2)
6His, N-terminus (1)
GST (1)
His (6)
His, N-Terminal (1)
His-GST (1)
Myc-DDK (Flag) (3)
Human (17)
Mouse (6)
Rat (1)
293T Cells (1)
E. coli (19)
HEK 293 Cells (3)
High Five Cells (1)
Wheat Germ Extract (1)
Purified (7)
Low endotoxin level (14)
Biologically active (3)
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High Five Cells
His, N-Terminal
Biologically active
Less than 0.1 ng/µg of protein (less than 1EU/µg).
15 kDa
5 µg/$268; 25 µg/$349
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E. coli
None
Less than 0.2 EU/µg protein (determined by LAL method).
12.5 kDa (SDS-PAGE)
50 µg/$364; 10 µg/$278; 1 mg/$1,612
MIF Protein
Select
E. coli
His
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$317; 500 µg/$526; 20 µg/$245
Select
E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
~15 kDa
25 µg/$291; 100 µg/$452; 1 mg/$1,587
MIF Protein
Select
E. coli
None
Biologically active
Less than 1.0 EU/µg protein (determined by LAL method).
~15 kDa
25 µg/$282; 100 µg/$452; 1 mg/$1,539
MIF Protein
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
100 µg/$411; 500 µg/$985; 20 µg/$276
MIF Protein - Purified recombinant protein MIF was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
12.3 kDa
20 µg/$1,107
MIF Protein - Recombinant Macrophage Migration Inhibitory Factor By SDS-PAGE
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E. coli
His
13.3 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
MIF Protein - Recombinant Macrophage Migration Inhibitory Factor By SDS-PAGE
Select
E. coli
His-GST
44.2 kDa
10 µg/$282; 50 µg/$450; 100 µg/$717; 200 µg/$897; 1 mg/$2,010; 500 µg/$1,547; 5 mg/$3,137; 2 mg/$2,198
MIF Protein - Recombinant Macrophage Migration Inhibitory Factor By SDS-PAGE
Select
E. coli
His
13.4 kDa
10 µg/$282; 50 µg/$450; 100 µg/$717; 200 µg/$897; 1 mg/$2,010; 500 µg/$1,547; 5 mg/$3,137; 2 mg/$2,198
MIF Protein - Recombinant Macrophage Migration Inhibitory Factor By SDS-PAGE
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E. coli
His
13.3 kDa
10 µg/$285; 50 µg/$470; 100 µg/$750; 200 µg/$937; 1 mg/$2,063; 500 µg/$1,594; 5 mg/$3,277; 2 mg/$2,252
MIF Protein - 12.5% SDS-PAGE of human MIF stained with Coomassie Blue
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Wheat Germ Extract
GST
38.39 kDa
10 µg/$479; 25 µg/$670
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HEK 293 Cells
None
Less than 1.0 EU/µg protein (determined by LAL method).
50 µg/$395
MIF Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
12.3 kDa
20 µg/$150
MIF Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
12.3 kDa
100 µg/$494
Select
E. coli
None
Less than 0.1 EU/µg protein (determined by LAL method).
The 115 amino acid recombinant protein has a predicted molecular mass of approximately 12.5 kD. The protein migrates above 14 kD by SDS-PAGE in DTT-reducing conditions and above 12.5 kD in non-reducing conditions. The predicted N-terminal amino acid is Me
25 µg/$352; 100 µg/$731
Select
E. coli
None
Less than 0.1 EU/µg protein (determined by LAL method).
The 115 amino acid recombinant protein has a predicted molecular mass of approximately 12.5 kD. The protein migrates above 14 kD by SDS-PAGE in DTT-reducing conditions and above 12.5 kD in non-reducing conditions. The predicted N-terminal amino acid is Me
25 µg/$352; 100 µg/$731; 500 µg/$1,723
MIF Protein
Select
E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
MIF Protein
Select
E. coli
None
100 µg/$361; 500 µg/$739; 20 µg/$261
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
13.5 kDa
20 µg/$360; 100 µg/$728
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
12.5 kDa
20 µg/$360; 100 µg/$728
Select
E. coli
114 AA
His
Less than 0.1 EU/µg protein (determined by LAL method).
12.65 kDa
10 mg/$4,268; 5 mg/$3,201; 1 mg/$1,868; 0.5 mg/$1,475; 200 µg/$1,103; 100 µg/$756; 50 µg/$531
Select
E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
13.5kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
Select
E. coli
None
Less than 1.0 EU/µg protein (determined by LAL method).
12.5kD
10 µg/$321; 50 µg/$534; 500 µg/$1,680; 1 mg/$2,353
Select
E. coli
6His, N-terminus
13 kDa
10 µg/$265; 50 µg/$315
Viewing 1-25 of 25 product results


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PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).