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WT1 / Wilms Tumor 1

Wilms tumor 1

WT1 / Wilms Tumor 1 is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilms tumor. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation codon upstream of, and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated.

Gene Name: Wilms tumor 1
Synonyms: WT1, GUD, EWS-WT1, Amino-terminal domain of EWS, WAGR, WIT-2, Wilms tumor protein, AWT1, Wilms tumor 1, WT33, NPHS4, WT-1
Target Sequences: NM_024426 NP_077744.3 P19544

Publications (5)

1
Characterization of monoclonal antibodies directed to the amino-terminus of the WT1, Wilms' tumor suppressor protein. Rauscher FJ, Morris JF, Fredericks WJ, Lopez-Guisa J, Balakrishnan C, Jost M, Herlyn M, Rodeck U. Hybridoma. 1998 17:191-8. [PubMed:9627060]
2
WT1 protein directly regulates expression of vascular endothelial growth factor and is a mediator of tumor response to hypoxia. McCarty G, Awad O, Loeb DM. The Journal of biological chemistry. 2011 286:43634-43. [PubMed:22030397] [PMC:PMC3243556]
3
Mature induced-pluripotent-stem-cell-derived human podocytes reconstitute kidney glomerular-capillary-wall function on a chip. Musah S, , Mammoto A, Ferrante TC, Jeanty SSF, Hirano-Kobayashi M, Mammoto T, Roberts K, Chung S, Novak R, Ingram M, Fatanat-Didar T, Koshy S, Weaver JC, Church GM, , Ingber DE, ,. Nature biomedical engineering. 2017 1: (IHC; Human) [Full Text Article] [PubMed:29038743] Related Antibodies: LS-C224662.
4
Glomerular Aging and Focal Global Glomerulosclerosis: A Podometric Perspective. Hodgin JB, Bitzer M, Wickman L, Afshinnia F, Wang SQ, O'Connor C, Yang Y, Meadowbrooke C, Chowdhury M, Kikuchi M, Wiggins JE, Wiggins RC. Journal of the American Society of Nephrology : JASN. 2015 26:3162-78. (WB; Human) [Full Text Article] [PubMed:26038526]
5
Directed differentiation of human induced pluripotent stem cells into mature kidney podocytes and establishment of a Glomerulus Chip. Samira Musah, Nikolaos Dimitrakakis, Diogo M Camacho, George M Church, Donald E Ingber. Nature protocols. 2018 Jul;13:1662-1685. [Full Text Article] [PubMed:29995874] [PMC:PMC6701189] Related Antibodies: LS-C224662.
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Products
Proteins (15)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (7)
Recombinant (7)
WT1 / Wilms Tumor 1 (15)
No (15)
6His, N-terminus (4)
6His-SUMO, N-terminus (1)
GST (1)
GST, N-terminus (1)
His (1)
Myc-DDK (Flag) (6)
Human (12)
293T Cells (3)
E. coli (4)
HEK 293 Cells (5)
Wheat Germ Extract (2)
Yeast (1)
Purified (4)
WT1 / Wilms Tumor 1 Protein
Select
E. coli
6His-SUMO, N-terminus
65.2 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
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HEK 293 Cells
6His, N-terminus
36.8 kDa
100 µg/$298
WT1 / Wilms Tumor 1 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$446; 20 µg/$336; 1 mg/$1,460
WT1 / Wilms Tumor 1 Protein - 12.5% SDS-PAGE of human WT1 stained with Coomassie Blue
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Wheat Germ Extract
GST
60.9 kDa
2 µg/$439
WT1 / Wilms Tumor 1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
WT1 / Wilms Tumor 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
54.3 kDa
20 µg/$215
WT1 / Wilms Tumor 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
54.4 kDa
20 µg/$215
WT1 / Wilms Tumor 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
33.5 kDa
20 µg/$150
WT1 / Wilms Tumor 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
54.4 kDa
100 µg/$710
WT1 / Wilms Tumor 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
54.3 kDa
100 µg/$710
WT1 / Wilms Tumor 1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
33.5 kDa
100 µg/$494
WT1 / Wilms Tumor 1 Protein - Recombinant Wilms Tumor Protein (WT1) by SDS-PAGE
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E. coli
None
46.5kDa
10 µg/$246; 50 µg/$299; 200 µg/$442; 100 µg/$352; 1 mg/$1,242; 500 µg/$882; 5 mg/$2,097; 2 mg/$1,391
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E. coli
His
38.2 kD
1 mg/$1,355; 100 µg/$420; 20 µg/$323
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HEK 293 Cells
6His, N-terminus
36.8 kDa
200 µl/$283
WT1 / Wilms Tumor 1 Protein - SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
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E. coli
6His, N-terminus
36.8 kDa
50 µg/$315; 100 µg/$365
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).