Target
Human ABCB4 / MDR3
Synonyms
ABCB4 | ABC21 | ICP3 | GBD1 | MDR3 | MDR2 | Multidrug resistance protein 3 | Multiple drug resistance 3 | P-glycoprotein 3 | Pgy2 | PGY3 | PFIC-3 | MDR2/3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Ready-to-Use.
Also available
Concentrated.
Immunogen
Recombinant Human MDR3
Applications
- IHC (1:100 - 1:200)
- IHC - Paraffin (1:50)
- ELISA (1:100 - 1:200)
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Apply antibody directly. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee