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| Catalog Number | Size | Price |
|---|---|---|
| LS-B16324-0.05 | 0.05 ml | $460 |
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IHC‑plus™ Monoclonal Mouse anti‑Human ABCB4 / MDR3 Antibody (Concentrated, IHC) LS‑B16324
IHC‑plus™ Monoclonal Mouse anti‑Human ABCB4 / MDR3 Antibody (Concentrated, IHC) LS‑B16324
Note: This antibody replaces LS-C759824
Antibody:
ABCB4 / MDR3 Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
ABCB4 / MDR3 Mouse anti-Human Monoclonal (Concentrated) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Concentrated
Other formats:
Specifications
Description
MDR3 antibody LS-B16324 is an unconjugated mouse monoclonal antibody to human MDR3 (ABCB4). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human ABCB4 / MDR3
Synonyms
ABCB4 | ABC21 | ICP3 | GBD1 | MDR3 | MDR2 | Multidrug resistance protein 3 | Multiple drug resistance 3 | P-glycoprotein 3 | Pgy2 | PGY3 | PFIC-3 | MDR2/3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
Monoclonal
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Concentrated.
Also available Ready-to-Use.
Immunogen
Recombinant Human MDR3
Applications
- IHC (1:100 - 1:200)
- IHC - Paraffin (1:50)
- ELISA (1:100 - 1:200)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Dilute antibody to its recommended working dilution using the same buffer defined in the Presentation. Manual Use: 1. Pretreatment: Perform heat-induced epitope retrieval (HIER) at pH 9 for 10 to 30 minutes. 2. Peroxide Block: Block in peroxidase blocking solution for 5 minutes at room temperature. (Not required if using Alkaline Phosphatase System.) 3. Primary Antibody: Dilute antibody at 1:100-1:200 before applying. Incubate antibody for 10 to 30 minutes at room temperature. 4. Secondary Antibody: Incubate for 20 to 30 minutes at room temperature. 5. Substrate Development: Incubate DAB or Fast Red for 5 to 10 minutes at room temperature. 6. Counterstain: Counterstain with hematoxylin for 0.5 to 5 minutes, depending on the hematoxylin used. Rinse with distilled water and blueing solution for 30 seconds. 7. Dehydrate and apply coverslip.
Presentation
Tris, pH 7.3-7.7, 1% BSA, <0.1% Sodium Azide
Storage
Store at 2°C to 8°C. Do not freeze.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ABCB4 / MDR3
Validation
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
See More About...
LSBio Ratings
IHC-plus™ ABCB4 / MDR3 Antibody (Concentrated) for IHC, ELISA LS-B16324 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human
Applications:
IHC, IHC - Frozen, ICC, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC - Paraffin, Immunofluorescence, ELISA
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
Date Requested: 11/22/2024
