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Antibodies > AKT1 > LS-C782363
 
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Catalog Number Size Price
LS-C782363-100 100 µg (1 mg/ml) $574 
AKT1 Antibody - IHC testing of FFPE human pancreas with AKT1 antibody (clone AKT1/2552). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
AKT1 Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AKT1 antibody (clone AKT1/2552). These results demonstrate the foremost specificity of the AKT1/2552 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
AKT1 Antibody - SDS-PAGE analysis of purified, BSA-free AKT1 antibody (clone AKT1/2552) as confirmation of integrity and purity.
AKT1 Antibody - IHC testing of FFPE human pancreas with AKT1 antibody (clone AKT1/2552). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
AKT1 Antibody - Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using AKT1 antibody (clone AKT1/2552). These results demonstrate the foremost specificity of the AKT1/2552 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SDs) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SDs) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.
AKT1 Antibody - SDS-PAGE analysis of purified, BSA-free AKT1 antibody (clone AKT1/2552) as confirmation of integrity and purity.
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Monoclonal Mouse anti‑Human AKT1 Antibody (clone AKT1/2552, Azide‑free,BSA‑free, IHC) LS‑C782363

Monoclonal Mouse anti‑Human AKT1 Antibody (clone AKT1/2552, Azide‑free,BSA‑free, IHC) LS‑C782363

Submit Review LSBio Guarantee
Antibody:
AKT1 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (AKT1/2552) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free
Price
Catalog Number
$574
LS-C782363-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal (Azide-free, BSA-free) (AKT1/2552) Antibody
Application:
IHC-P
Reactivity:
Human
Format:
Unconjugated, Azide-free, BSA-free

Specifications

Description
AKT1 antibody LS-C782363 is an unconjugated mouse monoclonal antibody to human AKT1. Validated for IHC.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
AKT1/2552
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Azide-free, BSA-free
Immunogen
A portion of amino acids 85-189 from the human protein was used as the immunogen for the AKT1 antibody.
Specificity
This antibody has been extensively validated, including cross-reactivity testing against 19,000 full-length proteins by HuProt(TM) microarray analysis.
Applications
  • IHC - Paraffin (1 - 2 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Titering of the AKT1 antibody may be required for optimal performance.
Presentation
1X PBS
Storage
Aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Applications: IHC, Immunofluorescence, Western blot
AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS‑C154001
Species: Human, Chimpanzee, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa428‑457, Azide‑free, HRP) LS‑C251144
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa438‑468, Azide‑free, HRP) LS‑C275023
Species: Human
Applications: Western blot, ELISA
See all AKT1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
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