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Antibodies > AKT1 > LS-C755391
 
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Catalog Number Size Price
LS-C755391-50 50 µl (1 mg/ml) $303 
LS-C755391-100 100 µl (1 mg/ml) $365 
AKT1 Antibody - ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with AKT1/3 antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
AKT1 Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: NIH3T3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human AKT1 Lane 2.: Recombinant Human AKT2 Lane 3.: Recombinant Human AKT3
AKT1 Antibody - ICC/IF analysis of AKT1/3 in A549 cells. The cell was stained with AKT1/3 antibody (1:100).The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).
AKT1 Antibody - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: NIH3T3 cell lysate The recombinant proteins (50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human AKT1/3 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: Recombinant Human AKT1 Lane 2.: Recombinant Human AKT2 Lane 3.: Recombinant Human AKT3
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Monoclonal Mouse anti‑Human AKT1 Antibody (clone AT6D5, WB) LS‑C755391

Monoclonal Mouse anti‑Human AKT1 Antibody (clone AT6D5, WB) LS‑C755391

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Antibody:
AKT1 Mouse anti-Human Monoclonal (AT6D5) Antibody
Application:
ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$303
LS-C755391-50
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal (AT6D5) Antibody
Application:
ICC, WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C755391 is an unconjugated mouse monoclonal antibody to human AKT1. Validated for ELISA, Flow, ICC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
AT6D5
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Recombinant human AKT1 (1-480aa) purified from insect cell
Applications
  • ICC (1:100)
  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 10% Glycerol
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Applications: IHC, Immunofluorescence, Western blot
AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS‑C154001
Species: Human, Chimpanzee, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa428‑457, Azide‑free, HRP) LS‑C251144
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa438‑468, Azide‑free, HRP) LS‑C275023
Species: Human
Applications: Western blot, ELISA
See all AKT1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
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