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Antibodies > AKT1 > LS-C370913
 
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Catalog Number Size Price
LS-C370913-50 50 µg $294 
LS-C370913-100 100 µg $360 
AKT1 Antibody
AKT1 Antibody
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA15905A0Rb at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA15905A0Rb at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil tissue using AKT1 Antibody at dilution of 1:100
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human prostate tissue using AKT1 Antibody at dilution of 1:100
AKT1 Antibody - Immunofluorescence staining of Hela cells with AKT1 Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
AKT1 Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate All lanes: AKT1 antibody at 7.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 49 kDa Observed band size: 56, 49 kDa
AKT1 Antibody
AKT1 Antibody
AKT1 Antibody
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human prostate tissue using CSB-PA15905A0Rb at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA15905A0Rb at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human tonsil tissue using AKT1 Antibody at dilution of 1:100
AKT1 Antibody - Immunohistochemistry of paraffin-embedded human prostate tissue using AKT1 Antibody at dilution of 1:100
AKT1 Antibody - Immunofluorescence staining of Hela cells with AKT1 Antibody at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
AKT1 Antibody - Western Blot Positive WB detected in: Hela whole cell lysate, A549 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate All lanes: AKT1 antibody at 7.4µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56, 49 kDa Observed band size: 56, 49 kDa
AKT1 Antibody
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Polyclonal Rabbit anti‑Human AKT1 Antibody (IHC) LS‑C370913

Polyclonal Rabbit anti‑Human AKT1 Antibody (IHC) LS‑C370913

Submit Review LSBio Guarantee
Antibody:
AKT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
FITC HRP Biotin
Price
Catalog Number
$294
LS-C370913-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ELISA, ChrIP
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
FITC HRP Biotin

Specifications

Description
AKT1 antibody LS-C370913 is an unconjugated rabbit polyclonal antibody to human AKT1. Validated for ChrIP, ELISA and IHC.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with FITC, HRP, Biotin.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Immunogen
Recombinant human RAC-alpha serine/threonine-protein kinase protein.
Specificity
Human AKT1
Applications
  • IHC
  • IHC - Paraffin
  • ELISA
  • Chromatin Immunoprecipitation
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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Featured Products

AKT1 Antibody - Anti-AKT1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
AKT1 Antibody (aa121‑170) LS‑B1510
Species: Human, Baboon, Frog
Applications: IHC, IHC - Paraffin, Western blot
Product
AKT1 Antibody (phospho‑Thr450, PE) LS‑C252845
Species: Human
Applications: ELISA, Dot Blot
Product
AKT1 Antibody (phospho‑Thr308) LS‑C122634
Species: Human, Mouse, Rat
Applications: IHC, Immunofluorescence, Western blot
AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS‑C154001
Species: Human, Chimpanzee, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa428‑457, Azide‑free, HRP) LS‑C251144
Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Product
AKT1 Antibody (aa438‑468, Azide‑free, HRP) LS‑C275023
Species: Human
Applications: Western blot, ELISA
See all AKT1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/15/2024
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