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Antibodies > AKT1 > LS-C812876
 
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Catalog Number Size Price
LS-C812876-50 50 µg $332 
LS-C812876-100 100 µg $404 
LS-C812876-200 200 µg $505 
AKT1 Antibody - L:Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments the same sample quality,and using DSBA mouse mAb (1:1000 diluted).R:Western blot detection of AKT in AKT1,AKT2 and AKT3 recombinant antigen fragments and using AKT (pan) mouse mAb (1:1000 diluted).
AKT1 Antibody - Western blot detection of total AKT in rat heart, rat testis, mouse brain, mouse spleen and C6 cell lysates and using AKT (pan) mouse mAb (1:1000 diluted). Predicted band size: 60KDa. Observed band size: 60KDa.
AKT1 Antibody - Immunoprecipitation analysis of Hela cell lysates using AKT (pan) mouse mAb.
AKT1 Antibody - L:Western blot detection of DSBA in AKT1,AKT2,AKT3 and DSBA recombinant antigen fragments the same sample quality,and using DSBA mouse mAb (1:1000 diluted).R:Western blot detection of AKT in AKT1,AKT2 and AKT3 recombinant antigen fragments and using AKT (pan) mouse mAb (1:1000 diluted).
AKT1 Antibody - Western blot detection of total AKT in rat heart, rat testis, mouse brain, mouse spleen and C6 cell lysates and using AKT (pan) mouse mAb (1:1000 diluted). Predicted band size: 60KDa. Observed band size: 60KDa.
AKT1 Antibody - Immunoprecipitation analysis of Hela cell lysates using AKT (pan) mouse mAb.
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Monoclonal Mouse anti‑Human AKT1 Antibody (WB) LS‑C812876

Monoclonal Mouse anti‑Human AKT1 Antibody (WB) LS‑C812876

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Antibody:
AKT1 Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$332
LS-C812876-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C812876 is an unconjugated mouse monoclonal antibody to AKT1 from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
Purified recombinant human AKT1 protein fragments expressed in E.coli.
Specificity
This antibody detects endogenous levels of total AKT and does not cross-react with related proteins.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
PBS, 0.02% Sodium Azide, 50% Glycerol, 0.5% BSA
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
AKT1 Antibody (Internal, clone 14E5.16C8.25F6) LS‑C154001
Species: Human, Chimpanzee, Mouse, Rat
Applications: IHC, Western blot, Flow Cytometry, ELISA
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Species: Human, Mouse
Applications: IHC, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
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Species: Human
Applications: Western blot, ELISA
See all AKT1 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/15/2024
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