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Antibodies > AKT1 > LS-C744820
 
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Catalog Number Size Price
LS-C744820-25 25 µl (1 mg/ml) $304 
AKT1 Antibody - Western Blot of Mouse anti-AKT Monoclonal Antibody. Lane 1: His-AKT1 Recombinant Protein. Lane 2: His-AKT2 Recombinant Protein. Lane 3: His-AKT3 Recombinant Protein. Load: 50 ng per lane. Primary antibody: Mouse anti-AKT Monoclonal Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 54-56 kDa, 54-56 kDa for AKT1, AKT2, AKT3.
AKT1 Antibody - Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
AKT1 Antibody - ELISA of Mouse Anti-AKT Antibody. Antigen: HCT-116 cell line. Coating amount: Confluent in the 96 well plate. Primary antibody: AKT or GAPDH antibody at 2 µg/mL. Dilution series: Primary and Secondary Antibodies 2-fold. Mid-point concentration: N/A. Secondary antibody: DyLight 680 donkey secondary antibody and DyLight 800 goat secondary antibody starting at 1:1,000. Substrate: None.
AKT1 Antibody - SDS PAGE of Mouse anti-AKT Monoclonal Antibody. Lane 1: Reduced Mouse anti-AKT Monoclonal Antibody. Lane 2: 3 µL Opal Prestained Marker Lane 3: Non-Reduced Mouse anti-AKT Monoclonal Antibody. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160kDa/Observed at 245 kDa; Reduced at 55, 25 kDa. Non-reduced migrates slightly higher.
AKT1 Antibody - Western Blot of Mouse anti-AKT Monoclonal Antibody. Lane 1: His-AKT1 Recombinant Protein. Lane 2: His-AKT2 Recombinant Protein. Lane 3: His-AKT3 Recombinant Protein. Load: 50 ng per lane. Primary antibody: Mouse anti-AKT Monoclonal Antibody at 1:1,000 overnight at 4°C. Secondary antibody: HRP mouse secondary antibody at 1:40,000 for 30 min at RT. Block: MB-070 for 30 min at RT. Predicted/Observed size: 54-56 kDa, 54-56 kDa for AKT1, AKT2, AKT3.
AKT1 Antibody - Western Blot of Mouse anti-AKT antibody. Lane 1: unstimulated NIH/3T3 cell lysates. Lane 2: PDGF stimulated NIH/3T3 cell lysates. Load: 10 µg per lane. Primary antibody: AKT antibody at 1:400 for overnight at 4°C. Secondary antibody: HRP conjugated Gt-a-Mouse IgG was used at a 1:40,000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent in TBS for 2h at RT. Observed size: ~56 kDa for AKT. Other band(s): none.
AKT1 Antibody - ELISA of Mouse Anti-AKT Antibody. Antigen: HCT-116 cell line. Coating amount: Confluent in the 96 well plate. Primary antibody: AKT or GAPDH antibody at 2 µg/mL. Dilution series: Primary and Secondary Antibodies 2-fold. Mid-point concentration: N/A. Secondary antibody: DyLight 680 donkey secondary antibody and DyLight 800 goat secondary antibody starting at 1:1,000. Substrate: None.
AKT1 Antibody - SDS PAGE of Mouse anti-AKT Monoclonal Antibody. Lane 1: Reduced Mouse anti-AKT Monoclonal Antibody. Lane 2: 3 µL Opal Prestained Marker Lane 3: Non-Reduced Mouse anti-AKT Monoclonal Antibody. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160kDa/Observed at 245 kDa; Reduced at 55, 25 kDa. Non-reduced migrates slightly higher.
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Monoclonal Mouse anti‑Human AKT1 Antibody (clone 14E5.16C8.25F6, Internal, IHC, WB) LS‑C744820

Monoclonal Mouse anti‑Human AKT1 Antibody (clone 14E5.16C8.25F6, Internal, IHC, WB) LS‑C744820

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Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (14E5.16C8.25F6) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$304
LS-C744820-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (14E5.16C8.25F6) Antibody
Application:
IHC, WB, Flo, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
AKT1 antibody LS-C744820 is an unconjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, Flow, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human, Chimpanzee, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Rat (at least 90% immunogen sequence identity)
Clonality
IgG Monoclonal
Clone
14E5.16C8.25F6
Conjugations
Unconjugated
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT Antibody was produced by repeated immunizations in mice with a synthetic peptide corresponding to residues internal to the human AKT1 protein.
Epitope
Internal
Specificity
This antibody is specific for human and mouse AKT protein. A BLAST analysis was used to suggest cross-reactivity with AKT1, AKT2, and AKT3 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT1, 2, 3 was determined with Western Blot. Cross reactivity of AKT from other sources has not been determined.
Applications
  • IHC (20 µg/ml)
  • Western blot (1:500 - 1:3000)
  • Flow Cytometry
  • ELISA (1:20000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT1
P31749 NM_005163 NP_005154.2

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AKT1 Antibody - Anti-AKT Monoclonal Antibody - Western Blot. anti-AKT Monoclonal Antibody in western blot shows detection of AKT (indicated by arrowhead at ~56 kD) on unstimulated (lane 1) and PDGF stimulated NIH/3T3 cell lysates (lane 2). Each lane contained approximately 10 ug of lysate. All samples were loaded on to a 4-20% gradient gel for separation. After electrophoresis, the gel was blocked with 5% BLOTTO (B501-0500 in TBS for 2h at RT. The membrane was probed with Anti-AKT antibody at a 1:1000 dilution in TBS with 5% BLOTTO, overnight at 4° C. For detection HRP conjugated Gt-a-Mouse IgG (p/n LS-C60680) was used at a 1:40000 dilution for 1 h at 4° C with FemtoMax enhanced chemiluminescent reagent (p/n FEMTOMAX-100). Images were captured using 2X2 binning for 10-20 sec using a BioSpectrum Imaging System (UVP Ltd.).
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Applications: IHC, Western blot, Flow Cytometry, ELISA
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 5/14/2024
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