Human AKT1

Mouse

Human (tested or 100% immunogen sequence identity)

Rat (at least 90% immunogen sequence identity)

IgG2b,k Monoclonal

5E5.F5.D7

Unconjugated

Protein A affinity chromatography

Unmodified. Also available Unmodified.

Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.

Internal

The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.

• IHC (20 µg/ml)
• Western blot (1:500 - 1:2000)
• Flow Cytometry
• ELISA (1:2000 - 1:10000)

The applications listed have been tested for the unmodified form of this product. Other forms have not been tested. Anti-AKT1 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kD in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2