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| Catalog Number | Size | Price |
|---|---|---|
| LS-C744942-50 | 50 µg (1 mg/ml) | $582 |
1 of 2
2 of 2
Monoclonal Mouse anti‑Human AKT1 Antibody (clone 5E5.F5.D7, RPE, Internal, IHC, IF, WB) LS‑C744942
Monoclonal Mouse anti‑Human AKT1 Antibody (clone 5E5.F5.D7, RPE, Internal, IHC, IF, WB) LS‑C744942
Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (RPE) (5E5.F5.D7) Antibody
Application:
IHC, IF, WB, Flo, ELISA, DB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
AKT1 Mouse anti-Human Monoclonal (Internal) (RPE) (5E5.F5.D7) Antibody
Application:
IHC, IF, WB, Flo, ELISA, DB
Reactivity:
Human, Mouse, Rat
Format:
R. Phycoerythrin, Unmodified
Specifications
Description
AKT1 antibody LS-C744942 is an RPE-conjugated mouse monoclonal antibody to AKT1 (Internal) from human. It is reactive with human, mouse and rat. Validated for DB, ELISA, Flow, IF, IHC and WB.
Target
Human AKT1
Synonyms
AKT1 | AKT | AKT-1 | C-AKT | Pan-AKT | PKB alpha | PKBalpha | Proto-oncogene c-Akt | Protein kinase B | Rac protein kinase alpha | PRKBA | Protein kinase B alpha | RAC-PK-alpha | PKB | PKB-ALPHA | RAC | RAC-ALPHA
Host
Mouse
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Clonality
IgG2a,k
Monoclonal
Clone
5E5.F5.D7
Conjugations
R. Phycoerythrin
Purification
Protein A affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein followed by monoclonal development.
Epitope
Internal
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. Anti-AKT1 antibody was purified from ascites by Protein A chromatography. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Applications
- IHC
- Immunofluorescence
- Western blot
- Flow Cytometry (1 µg/ml)
- ELISA
- Dot Blot
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About AKT1
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
Date Requested: 11/3/2024
