Human AKT1

Mouse

Human, Monkey, Mouse, Rat (tested or 100% immunogen sequence identity)

Chimpanzee, Mouse, Rat (at least 90% immunogen sequence identity)

IgG1,k Monoclonal

17F6.B11

Atto 594

Protein A affinity chromatography

Unmodified

Akt phospho S473 ATTO594 Conjugated Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.

pSer473

This antibody is specific for human and mouse AKT protein phosphorylated at S473. A BLAST analysis was used to suggest cross-reactivity with AKT pS473 from human, mouse, rat and chimpanzee sources based on 100% homology with the immunizing sequence. Cross-reactivity with AKT from other sources has not been determined. Cross-reactivity with AKT2 and AKT3 has not been determined.

• Immunofluorescence (1:5000)
• Western blot (1:10000)
• Flow Cytometry
• ELISA (1:20000)
• Fluorophore-Linked Immunosorbent Assay (1:20000)

Anti-AKT pS473 Antibody ATTO 594 Conjugated is designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (ELISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. Expect a band approximately 56 kD in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P31749 NCBI: NM_005163 NP_005154.2