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Catalog Number Size Price
LS-C758008-200 200 µl $393 
Alpha-Fetoprotein Antibody - AFP Antibody staining AFP in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Alpha-Fetoprotein Antibody - Anti-AFP Antibody at 1:500 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Alpha-Fetoprotein Antibody - Anti-AFP Antibody at 1:2000 dilution + human fetal Liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Alpha-Fetoprotein Antibody - AFP Antibody staining AFP in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Alpha-Fetoprotein Antibody - Anti-AFP Antibody at 1:500 dilution + HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Alpha-Fetoprotein Antibody - Anti-AFP Antibody at 1:2000 dilution + human fetal Liver lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-mouse IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 68 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Monoclonal Mouse anti‑Human Alpha‑Fetoprotein Antibody (clone 1087CT8.3.5, IHC, WB) LS‑C758008

Monoclonal Mouse anti‑Human Alpha‑Fetoprotein Antibody (clone 1087CT8.3.5, IHC, WB) LS‑C758008

Antibody:
Alpha-Fetoprotein Mouse anti-Human Monoclonal (1087CT8.3.5) Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C758008-200
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Alpha-Fetoprotein Mouse anti-Human Monoclonal (1087CT8.3.5) Antibody
Application:
IHC-P, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha-Fetoprotein antibody LS-C758008 is an unconjugated mouse monoclonal antibody to human Alpha-Fetoprotein. Validated for ELISA, IHC and WB.
Target
Human Alpha-Fetoprotein
Synonyms
AFP | Alpha-1-fetoprotein | Alpha-fetoprotein | HPAFP | Alpha-fetoglobulin | FETA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b,k Monoclonal
Clone
1087CT8.3.5
Conjugations
Unconjugated
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
This AFP antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 281-609 amino acids from human AFP.
Applications
  • IHC - Paraffin (1:25)
  • Western blot (1:500)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Western Blot Predicted Molecular Weight: 68.7 kDa
Presentation
PBS, 0.09% Sodium Azide
Storage
Short Term: Store at 2-8°C for up to 2 weeks. Long Term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Alpha-Fetoprotein
P02771 NM_001134 NP_001125.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024