Human Alpha-Fetoprotein

Rabbit

Human (tested or 100% immunogen sequence identity)

Polyclonal

FITC. Also available Unconjugated.

Ion exchange chromatography

Unmodified

Human alpha-1 fetoprotein

Human AFP / Alpha-Fetoprotein. The reactivity of the antiserum is restricted to alpha-fetoprotein. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against amniotic fluid, hepatoma sera and purified alpha-fetoprotein a single precipitin line is obtained. No precipitation reaction is obtained with normal human serum. Cross-reactivity The antiserum does not cross react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.

• Immunofluorescence
• Applications tested for the base form of this product only

This product is intended for use to identify and measure alpha fetoprotein at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from PBS, pH 7.2

Use 1 ml of sterile Distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02771 NCBI: NM_001134 NP_001125.1