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Antibodies > APCS / Serum Amyloid P / SAP > LS-C313414
 
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Catalog Number Size Price
LS-C313414-10 10 µg $318 
LS-C313414-100 100 µg (0.5 mg/ml) $470 
APCS / Serum Amyloid P / SAP Antibody - APCS / Serum Amyloid P / SAP antibody. IHC(P): Mouse Kidney Tissue.
APCS / Serum Amyloid P / SAP Antibody - Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB902) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 25KD. The expected band size for Serum Amyloid P is at 25KD.
APCS / Serum Amyloid P / SAP Antibody - Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 29KD. The expected band size for Serum Amyloid P is at 25KD.
APCS / Serum Amyloid P / SAP Antibody - WB of APCS / Serum Amyloid P / SAP antibody. All lanes: Anti-Serum Amyloid P at 0.5ug/ml. WB: Recombinant Mouse Serum Amyloid P Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD.
APCS / Serum Amyloid P / SAP Antibody - APCS / Serum Amyloid P / SAP antibody. IHC(P): Mouse Kidney Tissue.
APCS / Serum Amyloid P / SAP Antibody - Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody (PB902). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: mouse ovary tissue lysates, Lane 3: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody (Catalog # PB902) at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 25KD. The expected band size for Serum Amyloid P is at 25KD.
APCS / Serum Amyloid P / SAP Antibody - Western blot analysis of Serum Amyloid P using anti-Serum Amyloid P antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Serum Amyloid P antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Serum Amyloid P at approximately 29KD. The expected band size for Serum Amyloid P is at 25KD.
APCS / Serum Amyloid P / SAP Antibody - WB of APCS / Serum Amyloid P / SAP antibody. All lanes: Anti-Serum Amyloid P at 0.5ug/ml. WB: Recombinant Mouse Serum Amyloid P Protein 0.5ng. Predicted bind size: 39KD. Observed bind size: 39KD.
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Polyclonal Rabbit anti‑Mouse APCS / Serum Amyloid P / SAP Antibody (aa21‑224, IHC, WB) LS‑C313414

Polyclonal Rabbit anti‑Mouse APCS / Serum Amyloid P / SAP Antibody (aa21‑224, IHC, WB) LS‑C313414

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Antibody:
APCS / Serum Amyloid P / SAP Rabbit anti-Mouse Polyclonal (aa21-224) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C313414-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APCS / Serum Amyloid P / SAP Rabbit anti-Mouse Polyclonal (aa21-224) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
SAP antibody LS-C313414 is an unconjugated rabbit polyclonal antibody to mouse SAP (APCS / Serum Amyloid P) (aa21-224). Validated for ELISA, IHC and WB.
Target
Mouse APCS / Serum Amyloid P / SAP
Synonyms
APCS | 9.5S alpha-1-glycoprotein | Amyloid P component, serum | Pentaxin-related | PTX2 | Serum Amyloid P | Serum amyloid P-component | SAP
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived mouse Serum Amyloid P recombinant protein (Position: Q21-D224). Mouse Serum Amyloid P shares 70% and 80% amino acid (aa) sequences identity with human and rat Serum Amyloid P, respectively.
Epitope
aa21-224
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APCS / Serum Amyloid P / SAP
P02743 NM_001639 NP_001630.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/3/2024
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