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Antibodies > APG12 / ATG12 > LS-C156569
 
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Catalog Number Size Price
LS-C156569-400 400 µl $393 
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG12 / ATG12 Antibody - Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG12 / ATG12 Antibody - Western blot of anti-APG12L antibody in HeLa cell lysate. APG12L (arrow) was detected using the purified antibody.
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with Autophagy APG12L antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG12 / ATG12 Antibody - Fluorescent image of U251 cells stained with ATG12 antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG12 primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG12 immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.
APG12 / ATG12 Antibody - Western blot of anti-APG12L antibody in HeLa cell lysate. APG12L (arrow) was detected using the purified antibody.
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Polyclonal Rabbit anti‑Human APG12 / ATG12 Antibody (aa1‑30, IHC, IF, WB) LS‑C156569

Polyclonal Rabbit anti‑Human APG12 / ATG12 Antibody (aa1‑30, IHC, IF, WB) LS‑C156569

(4.0) Rating
Submit Review (1) Publication(s) LSBio Guarantee
Antibody:
APG12 / ATG12 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$393
LS-C156569-400
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
APG12 / ATG12 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Application:
IHC, IHC-P, IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ATG12 antibody LS-C156569 is an unconjugated rabbit polyclonal antibody to human ATG12 (APG12) (aa1-30). Validated for IF, IHC and WB. Cited in 1 publication.
(1) Publication(s)
Target
Human APG12 / ATG12
Synonyms
ATG12 | Autophagy-related protein 12 | APG12L | Autophagy related 12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Epitope
aa1-30
Specificity
This ATG12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ATG12.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:200)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, 0.09% Sodium Azide
Storage
Maintain refrigerated at 2°C to 8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG12 / ATG12
O94817 NM_004707 NP_004698.3

LSBio Ratings

APG12 / ATG12 Antibody (aa1-30) for IHC, IF/Immunofluorescence, WB/Western LS-C156569 has an LSBio Rating of
(4.0) Rating
Publications (4)

Publications (1)

Activation of autophagy in mesenchymal stem cells provides tumor stromal support. Sanchez CG, Penfornis P, Oskowitz AZ, Boonjindasup AG, Cai DZ, Dhule SS, Rowan BG, Kelekar A, Krause DS, Pochampally RR. Carcinogenesis. 2011 32:964-72.
PubMed: 21317300 PMC: PMC3128555
Submit a Publication for LS-C156569

Customer Reviews (0)

Submit a review for LS-C156569

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Applications: Immunofluorescence, Western blot, Immunoprecipitation, ELISA
APG12 / ATG12 Antibody - Anti-ATG12 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC‑plus™ APG12 / ATG12 Antibody (Internal) LS‑B2140
Species: Human, Mouse
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
APG12 / ATG12 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG12L Monoclonal Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
APG12 / ATG12 Antibody LS‑C156567
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
APG12 / ATG12 Antibody - ATG12 antibody Western blot of 721_B cell lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APG12 / ATG12 Antibody (C‑Terminus) LS‑C117261
Species: Human
Applications: Western blot
APG12 / ATG12 Antibody - Anti-ATG12 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
APG12 / ATG12 Antibody (aa79‑128, Biotin) LS‑C465784
Species: Rat, Human, Orangutan, Monkey, Mouse, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
Applications: IHC, IHC - Paraffin, Western blot
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Human EPO / Erythropoietin Protein (Recombinant His-GST) (aa28-193) - LS-G11249
Source: E. coli
Tag: His-GST
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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/22/2024
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Vector Laboratories, Inc.
6737 Mowry Ave
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United States

Tel: (800) 227-6666 / (650) 697-3600

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