Target
Human APG12 / ATG12
Synonyms
ATG12 | Autophagy-related protein 12 | APG12L | Autophagy related 12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
Synthetic peptide corresponding to amino acids at the N-Terminus of human Atg12.
Applications
- Immunofluorescence (10 µg/ml)
- Western blot (1 - 2 µg/ml)
Usage
Immunoblotting: use at 1-2ug/ml. Bands of ~48-55kDa, corresponding to Atg12-Atg5 complex, are detected. Note: the predicted molecular weight of Atg12 is ~15kDa. Immunofluorescence: use at 10ug/ml. These are recommended concentrations. Endusers should determine optimal concentrations for their applications.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Reconstitution
PBS or medium which is identical to that used in the assay system.
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee