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Catalog Number Size Price
LS-C776851-100 100 µg (1 mg/ml) $479 
APG12 / ATG12 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG12 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG12 Antibody (D) Composite.
APG12 / ATG12 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48 and 55kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~48 and 55kDa.
APG12 / ATG12 Antibody - Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG12 Polyclonal Antibody. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG12 Antibody (D) Composite.
APG12 / ATG12 Antibody - Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~48 and 55kDa ATG12 protein using Rabbit Anti-ATG12 Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human HeLa (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG12 Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~48 and 55kDa.
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Polyclonal Rabbit anti‑Human APG12 / ATG12 Antibody (PerCP, IF, WB) LS‑C776851

Polyclonal Rabbit anti‑Human APG12 / ATG12 Antibody (PerCP, IF, WB) LS‑C776851

Antibody:
APG12 / ATG12 Rabbit anti-Human Polyclonal (PerCP) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:
Price
Catalog Number
$479
LS-C776851-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
APG12 / ATG12 Rabbit anti-Human Polyclonal (PerCP) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Peridinin-chlorophyll-protein, Unmodified
Other formats:

Specifications

Description
ATG12 antibody LS-C776851 is a PerCP-conjugated rabbit polyclonal antibody to human ATG12 (APG12). Validated for IF and WB.
Target
Human APG12 / ATG12
Synonyms
ATG12 | Autophagy-related protein 12 | APG12L | Autophagy related 12 | FBR93 | APG12 | APG12-like | Ubiquitin-like protein ATG12 | HAPG12
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Peridinin-chlorophyll-protein. Also available conjugated with APC, Atto 390, Atto 488, Atto 594, Biotin, FITC, RPE.
Purification
Peptide Affinity Purified
Modifications
Unmodified
Immunogen
Synthetic peptide from the N-Terminus of human ATG12
Specificity
Predicted molecular weight at ~15.1 kDa. Observed molecular weights at ~48-55 kDa based on ATG12-ATG5 heterodimer.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Applications tested for the base form of this product only
Usage
Applications should be user optimized.
Presentation
PBS, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About APG12 / ATG12
O94817 NM_004707 NP_004698.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/3/2024