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Catalog Number Size Price
LS-C19118-100 100 µg (0.5 mg/ml) $567 
ATAD5 / ELG1 Antibody - Anti-Elg1 Antibody - Western Blot. Western blot of Affinity Purified anti-Elg1 antibody shows detection of a band ~120 kD corresponding to human Elg1 (arrowhead) in various cell lysates. Lanes contain ~5 ug of HeLa nuclear extract (1), HeLa (2), A431 (3), Jurkat (4) and HEK293 (5) whole cell lysates. After SDS-PAGE, transfer and blocking, the membrane was probed with the primary antibody diluted to 1:500. The membrane was then washed and reacted with a HRP conjugated Gt-a-Rabbit IgG [H&L] MX followed by ECL detection using a 2 m exposure time. The expected molecular weight of Elg1 is 120kD according to Kanellis P et al. 2003, although the predicted molecular weight is 207 kD. The 50kD bands in Jurkat and 293 cell lysates are probably cross-reaction with other proteins. Both the 120 kD and 50 kD bands are not observed when antibody is pre-incubated with peptide (data not shown).

Polyclonal Rabbit anti‑Human ATAD5 / ELG1 Antibody (WB) LS‑C19118

Polyclonal Rabbit anti‑Human ATAD5 / ELG1 Antibody (WB) LS‑C19118

Antibody:
ATAD5 / ELG1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$567
LS-C19118-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
ATAD5 / ELG1 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ELG1 antibody LS-C19118 is an unconjugated rabbit polyclonal antibody to human ELG1 (ATAD5). Validated for ELISA and WB. Cited in 1 publication.
Target
Human ATAD5 / ELG1
Synonyms
ATAD5 | C17orf41 | FRAG1 | ELG1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 63-76 of Human Elg 1.
Specificity
A BLAST analysis of the immunogen suggest full to partial reactivity with several hypothetical proteins. This is not unexpected for such a novel protein. The following proteins should be considered to be highly homologous to Elg1 when using this antibody: human chromosome fragility associated gene 1 (NP_079133), hypothetical dog protein FLJ12735 (XP_548276), hypothetical chimpanzee protein FLJ12735 (XP_511388), and a hypothetical human protein of 1224 residues (CAH10412). All show 100% homology to the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:3000 - 1:15000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 120 kD in size corresponding to Elg1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ATAD5 / ELG1
Q96QE3 NM_024857 NP_079133.3

LSBio Ratings

ATAD5 / ELG1 Antibody for WB/Western, ELISA LS-C19118 has an LSBio Rating of
Publications (4)

Publications (1)

Patient-derived iPSC-cerebral organoid modeling of the 17q11.2 microdeletion syndrome establishes CRLF3 as a critical regulator of neurogenesis. Michelle L Wegscheid, Corina Anastasaki, Kelly A Hartigan, Olivia M Cobb, Jason B Papke, Jennifer N Traber, Stephanie M Morris, David H Gutmann. Cell reports. 2021 July;36:109315.

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/3/2024