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Catalog Number Size Price
LS-C392383-20 20 µg (0.2 mg/ml) $371 
LS-C392383-100 100 µg (0.2 mg/ml) $574 
BAX Antibody - Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336).  This image was taken for the unmodified form of this product. Other forms have not been tested.
BAX Antibody - Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa.
BAX Antibody - Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with anti-Bax antibody (Clone SPM336).  This image was taken for the unmodified form of this product. Other forms have not been tested.
BAX Antibody - Western blot testing of HeLa cell lysate with anti-Bax antibody (clone SPM336). Observed molecular weight ~21kDa.
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Monoclonal Mouse anti‑Human BAX Antibody (clone SPM336, aa3‑16, IHC, IF, WB) LS‑C392383

Monoclonal Mouse anti‑Human BAX Antibody (clone SPM336, aa3‑16, IHC, IF, WB) LS‑C392383

Antibody:
BAX Mouse anti-Human Monoclonal (aa3-16) (SPM336) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Monkey, Bovine, Dog, Goat, Pig
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$371
LS-C392383-20
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
BAX Mouse anti-Human Monoclonal (aa3-16) (SPM336) Antibody
Application:
IHC, IHC-P, IF, WB, Flo
Reactivity:
Human, Monkey, Bovine, Dog, Goat, Pig
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
BAX antibody LS-C392383 is an unconjugated mouse monoclonal antibody to BAX (aa3-16) from human. It is reactive with human, bovine, dog and other species. Validated for Flow, IF, IHC and WB.
Target
Human BAX
Synonyms
BAX | BCL2-associated X protein | Bcl2-L-4 | Apoptosis regulator BAX | Bcl-2-like protein 4 | BCL2L4
Host
Mouse
Reactivity
Human, Monkey, Bovine, Dog, Goat, Pig (tested or 100% immunogen sequence identity)
Non-Reactivity
Mouse, Rat
Clonality
IgG1 Monoclonal
Clone
SPM336
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified. Also available Azide-free, BSA-free.
Immunogen
Synthetic peptide (GSGEQPRGGGPTSS, aa3-16 of UniProt Q07812) from the human protein. Percent identity by BLAST analysis: Human, Ferret, Cat, Bovine, Goat, Pig, Dog (100%); Bat, Elephant, Opossum (93%); Rat, Hamster, Guinea pig (86%); Mouse (79%).
Epitope
aa3-16
Specificity
Human BAX
Applications
  • IHC
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Immunofluorescence (1 - 2 µg/ml)
  • Western blot (0.5 - 1 µg/ml)
  • Flow Cytometry (0.5 - 1 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Recognizes a protein of 21kDa, identified as the Bax protein. This mAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2. The optimal dilution of the anti-Bax antibody for each application should be determined by the researcher. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.
Presentation
1X PBS, 0.1 mg/ml BSA, 0.05% sodium azide
Storage
Store at 2°C to 8°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About BAX
Q07812 NM_004324 NP_004315.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 10/31/2024